Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis
Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis
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DOI：
10.1038/nbt1290
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发表时间：
2007-03-01
影响因子：
46.9
通讯作者：
Richardson, Paul M.
中科院分区：
文献类型：
--
作者：
Jeffries, Thomas W.;Grigoriev, Igor V.;Richardson, Paul M.
木糖是植物木质纤维素的主要成分，其发酵对植物生物质转化为燃料和化学品具有重要意义。毕赤酵母是一种经过充分研究的天然木糖发酵酵母。木糖代谢的机制和调控已被研究清楚，木糖代谢基因已被用于酿酒酵母的木糖代谢工程。我们已经测序并组装了弓形虫的全基因组。序列数据揭示了基因组组织的不同寻常方面，许多生物转化基因，对中心代谢途径调控的初步见解以及具有相关功能的共定位基因的几个例子。基因组序列提供了如何调节其氧化还原平衡，同时非常有效地发酵木糖在微氧条件下的见解。
Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.