Cytotoxicity of root perforation repair materials.
Cytotoxicity of root perforation repair materials.
复制标题
根穿孔修复材料的细胞毒性。
DOI：
10.1016/s0099-2399(98)80050-2
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发表时间：
1998
影响因子：
4.2
通讯作者：
N. Ewoldsen
中科院分区：
文献类型：
--
作者：
H. Makkawy;S. Koka;M. T. Lavin;N. Ewoldsen
必须研究牙科修复材料的细胞毒性，以确保安全的生物反应。MTS试验是一种基于培养细胞线粒体活性的有效可靠的细胞活力测定方法，用于评价两种树脂改性玻璃离子粘固剂（R-M GIC）（Fuji Duet和Fuji II LC，GC America，芝加哥，IL）和一种牙科汞合金（Contour，Caulk，约克，PA）对人牙周膜（PDL）细胞的影响-所有材料均建议用于根穿孔修复。每种材料制备12个4 × 6 mm圆柱体，并置于5 ml补充有100 μ g/ml青霉素、50 μ g/ml庆大霉素和5%胎牛血清的α-最小必需培养基中24、48和72 h（n = 3）。将100 μ l的PBS转移到含有PDL细胞的一式三份威尔斯孔中，所述PDL细胞先前以10，000个细胞/孔的密度接种在96孔板中，并在37 ℃下用5%二氧化碳孵育24小时。α-最低必需培养基（含补充剂）提供了基线数据。根据制造商说明书测定与活细胞数成正比的490 nm处的光密度。方差分析用于检测治疗之间的差异，Tukey's HSD（p < 0.05）用于检测组平均值之间的差异。结果表明，材料和时间都影响细胞活力（p < 0.0001），与对照和其他两种测试材料相比，汞合金在24 h时显著抑制细胞活力。在48和72小时，所有三种材料对细胞活力表现出相似的轻微抑制作用。使用树脂改性玻璃离子水门汀作为根穿孔修复材料最初（<24小时）可能会导致PDL细胞比测试的牙科汞合金更有利的反应。
The cytotoxicity of restorative dental materials must be investigated to ensure a safe biological response. The MTS assay, a valid and reliable measure of cell viability based on the mitochondrial activity of cultured cells, was used to evaluate the effects on human periodontal ligament (PDL) cells of two resin-modified glass ionomer cements (R-M GICs) (Fuji Duet and Fuji II LC, GC America, Chicago, IL) and one dental amalgam (Contour, Caulk, York, PA)—all suggested materials for root perforation repair. Twelve 4 × 6 mm cylinders of each material were fabricated and placed in 5 ml of α-minimum essential medium supplemented with 100 g/ml of penicillin, 50 g/ml of gentamicin, and 5% fetal bovine serum for 24, 48, and 72 h (n = 3). One hundred microliters of eluate was transferred to triplicate wells containing PDL cells previously plated at a density of 10,000 cells/well in a 96-well plate, and incubated for 24 h at 37°C with 5% carbon dioxide. α-Minimum essential medium with supplements provided baseline data. Optical density at 490 nm, directly proportional to the number of viable cells, was determined according to manufacturer instructions. Analysis of variance was used to detect differences between treatments and Tukey's HSD (p < 0.05) to detect for differences between group means. Results demonstrated that both material and time affected cell viability (p < 0.0001), with amalgam eluate significantly inhibitory on cell viability at 24 h, compared with control and the two other tested materials. At 48 and 72 h, all three materials exhibited a similar slightly inhibitory effect on the cell viability. Use of resin-modified glass ionomer cement as a root perforation repair material initially (<24 h) may result in a more favorable response by PDL cells than the tested dental amalgam.