Structural analysis of the interaction between the SNARE Tlg1 and Vps51
Structural analysis of the interaction between the SNARE Tlg1 and Vps51
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DOI：
10.1111/j.1600-0854.2005.00374.x
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发表时间：
2006-02-01
期刊：
影响因子：
4.5
通讯作者：
Pelham, HRB
中科院分区：
文献类型：
--
作者：
Fridmann-Sirkis, Y;Kent, HM;Pelham, HRB
细胞中的膜融合涉及SNARE蛋白在并置膜上的相互作用。SNARE复合物的形成之前是拴系事件，并且已经鉴定了许多被认为介导这一过程的蛋白质复合物。VFT或GARP复合物是酵母中内体-高尔基体运输所必需的。它由四个亚基组成，其中之一，Vps 51，已被证明特异性结合SNARE Tlg 1，参与相同的融合事件。我们已经确定的N-末端结构域的Tlg 1结合到一个肽从N末端的Vps 51。结合主要取决于Vps 51的残基18-30。这些形成一个短螺旋，位于Tlg 1形成的三螺旋束中的保守凹槽中。令人惊讶的是，尽管Vps 51和Tlg 1都是从内体运输到晚期高尔基体所必需的，但从Vps 51中去除Tlg 1结合序列并不能阻止体内这种运输。因此，这种特殊的相互作用对囊泡对接或融合的过程不是至关重要的。
Membrane fusion in cells involves the interaction of SNARE proteins on apposing membranes. Formation of SNARE complexes is preceded by tethering events, and a number of protein complexes that are thought to mediate this have been identified. The VFT or GARP complex is required for endosome-Golgi traffic in yeast. It consists of four subunits, one of which, Vps51, has been shown to bind specifically to the SNARE Tlg1, which participates in the same fusion event. We have determined the structure of the N-terminal domain of Tlg1 bound to a peptide from the N terminus of Vps51. Binding depends mainly on residues 18-30 of Vps51. These form a short helix which lies in a conserved groove in the three-helix bundle formed by Tlg1. Surprisingly, although both Vps51 and Tlg1 are required for transport to the late Golgi from endosomes, removal of the Tlg1-binding sequences from Vps51 does not block such traffic in vivo. Thus, this particular interaction cannot be crucial to the process of vesicle docking or fusion.