Endogenous substrates for cyclic AMP-dependent and calcium-dependent protein phosphorylation in rabbit peritoneal neutrophils.
Endogenous substrates for cyclic AMP-dependent and calcium-dependent protein phosphorylation in rabbit peritoneal neutrophils.
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兔腹膜中性粒细胞中环 AMP 依赖性和钙依赖性蛋白质磷酸化的内源底物。
DOI：
10.1016/0304-4165(83)90133-2
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Becker,EL
中科院分区：
文献类型：
--
作者：
Huang,CK;HillJr,JM;Bormann,BJ;Mackin,WM;Becker,EL
与其他细胞一样，兔腹膜中性粒细胞中也存在camp依赖性（蛋白激酶A）和钙依赖性蛋白激酶。兔腹膜中性粒细胞胞浆中蛋白激酶A的主要底物是一种43 kDa的蛋白，似乎是肌动蛋白（pI5.7）。胞质中蛋白激酶A的其他底物是分子量为135000 （pI4.6）和13000 （pI4.8）的酸性很强的蛋白质。兔腹膜中性粒细胞中存在两类钙依赖性蛋白激酶：一类是钙，钙调素依赖性，另一类是钙，磷脂酰丝氨酸依赖性。磷脂酰丝氨酸在刺激钙依赖性蛋白激酶活性方面似乎比钙调素更有效。磷脂敏感的，钙依赖的蛋白激酶（蛋白激酶C），只存在于细胞质部分，表现出比来自相同来源的camp依赖的蛋白激酶高得多的活性。在细胞质中鉴定出蛋白激酶C的至少4个底物（mr13 000 （pI4.6）、43 000 （pI4.8）、41 000 （pI6.3）和34 000）。三氟拉嗪，一种抑制兔腹膜中性粒细胞的脱粒、聚集和刺激氧消耗的化合物。（Alobaidi， T., Naccache， P.H.和Sha'afi， R.I., 1981）《生物化学》。Biophys。Acta 675, 316-321)，也抑制蛋白激酶c的活性。camp依赖性和钙依赖性磷酸化系统在中性粒细胞功能中的可能作用进行了讨论。
As in other cells, cAMP-dependent (protein kinase A) and calcium-dependent protein kinases are present in the rabbit peritoneal neutrophil. The major substrates for protein kinase A in the cytosol of rabbit peritoneal neutrophil is a 43 kDa protein which appears to be actin (pI5.7). The other substrates for protein kinase A in the cytosol are very acidic proteins with molecular weights of 135 000 (pI4.6) and 130 000 (pI4.8). Two classes of calcium-dependent protein kinases are present in the rabbit peritoneal neutrophil: one is calcium, calmodulin-dependent, the other is calcium, phosphatidylserine-dependent. Phosphatidylserine appears to be much more effective than calmodulin in stimulting calcium-dependent protein kinase activity. The phospolipid-sensitive, calcium-dependent protein kinase (protein kinase C), present only in the cytosol fraction, exhibits much higher activity than the cAMP-dependent protein kinase from the same source. At least four substrates (Mr130 000 (pI4.6) 43 000 (pI4.8), 41 000 (pI6.3) and 34 000) of the protein kinase C in the cytosol were identified. Trifluoperazine, a compound which inhibits the degranulation, aggregation and stimulated oxygen consumption of rabbit peritoneal neutrophils. (Alobaidi, T., Naccache, P.H. and Sha'afi, R.I. (1981) Biochim. Biophys. Acta 675, 316–321), also inhibits the activity of protein kinase C. The possible role of cAMP-dependent and calcium-dependent phosphorylation system in neutrophil function is discussed.