A GLUCOSE-TRANSPORT PROTEIN EXPRESSED PREDOMINATELY IN INSULIN-RESPONSIVE TISSUES
A GLUCOSE-TRANSPORT PROTEIN EXPRESSED PREDOMINATELY IN INSULIN-RESPONSIVE TISSUES
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DOI：
10.1073/pnas.86.8.2535
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发表时间：
1989-04-01
影响因子：
11.1
通讯作者：
LODISH, HF
中科院分区：
文献类型：
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作者：
CHARRON, MJ;BROSIUS, FC;LODISH, HF
利用与大鼠脑葡萄糖转运蛋白（GT）的低严格性杂交，从大鼠比目鱼肌gt 10 cDNA文库中分离出2489个碱基对的cDNA克隆。它编码一个509个氨基酸的转运蛋白，其序列和预测的膜结构与大鼠脑和肝GT非常相似。肌肉GT样蛋白在氨基酸序列上与大鼠脑GT有65%相同，与大鼠肝GT有52%相同;主要差异在于NH 2-和COOH-末端亲水性片段。这种GT样mRNA主要在葡萄糖转运对胰岛素敏感的组织中表达，包括横纹肌、心肌和脂肪组织;在平滑肌和肾脏mRNA中也检测到低水平表达。该GT样cDNA是迄今为止鉴定的哺乳动物GT相关基因家族的第四个成员。我们建议它编码胰岛素敏感的GT。
Using low-stringency hybridization to the rat brain glucose transporter (GT), a 2489-base-pair cDNA clone was isolated from a rat soleus .lambda.gt10 cDNA library. It encodes a 509-amino acid portein whose sequence and predicted membrane structure is very similar to those of the rat brain and liver GTs. The muscle GT-like protein is 65% identical in amino acid sequence to the rat brain GT and 52% identical to the rat liver GT; the major difference are in the NH2- and the COOH-terminal hydrophilic segments. This GT-like mRNA is expressed predominately in tissues where glucose transport is sensitive to insulin, including striated muscle, cardiac muscle, and adipose tissue; low-level expression is also detected in smooth muscle and kidney mRNA. This GT-like cDNA is the fourth member of the mammalian GT-related gene family identified to date. We propose that it encodes an insulin-sensitive GT.