Electrochemical performance of biocathode microbial fuel cells using petroleum-contaminated soil and hot water spring
Electrochemical performance of biocathode microbial fuel cells using petroleum-contaminated soil and hot water spring
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DOI：
10.1007/s13762-018-1757-0
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发表时间：
2019-03
影响因子：
3.1
通讯作者：
Zargona Zafar;K. Ayaz;M. Nasir;Sameen Yousaf;Iqra Sharafat;Naeem Ali
中科院分区：
文献类型：
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作者：
Zargona Zafar;K. Ayaz;M. Nasir;Sameen Yousaf;Iqra Sharafat;Naeem Ali
微生物燃料电池是一种新兴的能源生产技术（电能和气体），利用潜在的电化学活性细菌降解不需要的污染物。在生物阴极微生物燃料电池（MFC）中，对产电[石油污染土壤（PCS）和温泉水（HSW）]和电营养化[活性污泥]细菌群落进行了富集和评估。环境样品的分子系统发育（454焦磷酸测序）分析显示，第二阶段富集后细菌密度和多样性发生了总体变化。在MFC-1 （PCS）和MFC-2 （HSW）反应器中，在阳极生物膜上生长的优势电生细菌属于变形菌门（80-98%）。富集后，阳极表面的细菌种类变化最大的是嗜麦芽寡养单胞菌（89%）和雪氏菌（shewanellasp）。（15%）。总体而言，在两种燃料电池的阴极生物膜上，铜绿假单胞菌的相对丰度（27-30%）最大。扫描电子显微镜和激光共聚焦扫描显微镜显示，生物膜的正极表面覆盖着不同的微菌落和分散的细菌细胞。循环伏安法（-1 ~ 1 V vs. Ag/AgCl）进一步证实了高效产电细菌的存在，它们能在MFC-1中产生≥8 mA的高电流，在MFC-2中产生≤0.37 y的高电流。在富集阶段2，MFC-1在电流密度为100 mA m−2（550 Ω）时记录了5500 mW m−2的最大功率密度；然而，它（Pmax= 1201 mW m2）在MFC-2中仍然低78%。傅里叶变换红外光谱和COD去除率[86% (SD = 8.3±2.0)]证实了MFC-1在运行过程中对石油污染物的有效降解。
Microbial fuel cells is growing technology for energy production (electrical and gaseous) with potential electrochemically active bacteria from degradation of unwanted contaminants. Electrogenic [petroleum-contaminated soil (PCS) and hot spring water HSW)] and electrotrophic [activated sludge] bacterial communities were enriched and evaluated for electric current production in biocathode microbial fuel cells (MFC). Molecular phylogenetic (454 pyrosequencing) analysis of environmental samples revealed an overall change in bacterial density and diversity after second-stage enrichment. The predominant electrogenic bacteria grown at anodic biofilms belonged to phylum Proteobacteria (80–98%) in both MFC-1 (PCS) and MFC-2 (HSW) reactors. After enrichment, the major shift in the bacterial species on anodic surface was observed in case ofStenotrophomonas maltophilia(89%) andshewanellasp. (15%) in the respective reactors. Overall, among electrotrophic bacteria, the relative abundance (27–30%) ofPseudomonas aeruginosawas maximum on the cathodic biofilm in both fuel cells. Scanning electron and confocal laser scanning microscopies of biofilms revealed that anode and cathode surfaces were covered with different microcolonies and dispersed bacterial cells. Cyclic voltammetry (− 1 to 1 V vs. Ag/AgCl) further confirmed the presence of highly proficient electrogenic bacteria capable of generating high electricity ranging from ≥ 8 mA in MFC-1 and ≤ 0.37-Y in MFC-2. Maximum power density of 5500 mW m−2at a current density of 100 mA m−2(550 Ω)] was recorded in MFC-1 during enrichment stage 2; however, it (Pmax= 1201 mW m2) remained 78% lower in MFC-2. Fourier transform infrared spectroscopy and COD removal [86% (SD = 8.3 ± 2.0)] of anolyte (PCS) confirmed active degradation of petroleum contaminants during the operation of MFC-1.