Simultaneous determination of five flavonoids in licorice using pressurized liquid extraction and capillary electrochromatography coupled with peak suppression diode array detection
Simultaneous determination of five flavonoids in licorice using pressurized liquid extraction and capillary electrochromatography coupled with peak suppression diode array detection
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DOI：
10.1016/j.chroma.2009.08.034
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发表时间：
2009-10-23
影响因子：
4.1
通讯作者：
Wang, Y. T.
中科院分区：
文献类型：
--
作者：
Chen, X. J.;Zhao, J.;Wang, Y. T.
建立了加压液体萃取（PLE）和毛细管电色谱（CEC）方法，以黄芩素为内标（IS），同时测定甘草中甘草苷、异甘草苷、ononin、甘草素和异甘草素5种黄酮类化合物的含量。由于异甘草苷的分辨率较差，因此使用峰抑制技术对 ononin 进行定量。分析在 Hypersil C-18 毛细管（3 μ m，100 μ m/25 cm）上进行，使用 10 mM 磷酸盐缓冲液（pH 3.0）/ACN（65:35，v/v）混合物作为流动相，在 25 W 和 30°C 下运行。检测波长设置为 275 nm（不含甘草苷和甘草素的参考波长）、360 nm（不含甘草苷和甘草素的参考波长）。异甘草苷和异甘草素的参考波长）和 254 nm（紫甘草苷的参考波长为 405 nm）。所有校准曲线在测试范围内均表现出良好的线性（R-2 > 0.9993）。 LOD和LOQ分别低于2.1和8.3μg/mL。 5种分析物相对峰面积的日内和日间RSD分别小于3.8和4.7%，回收率为98.2-103.8%。该方法经验证成功应用于甘草中5种黄酮类化合物的定量分析，有助于其质量控制。 (C) 2009 Elsevier B.V. 保留所有权利。
Pressurized liquid extraction (PLE) and capillary electrochromatography (CEC) methods were developed for the simultaneous determination of five flavonoids, namely liquiritin, isoliquiritin, ononin, liquiritigenin and isoliquiritigenin, in licorice using baicalein as internal standard (IS). Peak suppression technique was used for the quantification of ononin because of its poor resolution with isoliquiritin. The analysis was performed on a Hypersil C-18 capillary (3 mu m, 100 mu m/25 cm) with a mixture of 10 mM phosphate buffer (pH 3.0)/ACN (65:35, v/v) as mobile phase running at 25 W and 30 degrees C. The detection wavelengths were set at 275 nm (without reference wavelength for liquiritin and liquiritigenin), 360 nm (without reference wavelength for isoliquiritin and isoliquiritigenin) and 254 nm (with reference wavelength of 405 nm for ononin). All calibration curves showed good linearity (R-2 > 0.9993) within the test ranges. The LOD and LOQ were lower than 2.1 and 8.3 mu g/mL, respectively. The RSDs of intra- and interday for relative peak areas of five analytes to IS were less than 3.8 and 4.7%, respectively, and the recoveries were 98.2-103.8%. The validated method was successfully applied to the quantitative analysis of five flavonoids in licorice, which is helpful to its quality control. (C) 2009 Elsevier B.V. All rights reserved.