De Novo Expression of the Neurokinin 1 Receptor in Spinal Lamina I Pyramidal Neurons in Polyarthritis
De Novo Expression of the Neurokinin 1 Receptor in Spinal Lamina I Pyramidal Neurons in Polyarthritis
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DOI：
10.1002/cne.22024
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发表时间：
2009-05-20
影响因子：
2.5
通讯作者：
Ribeiro-Da-Silva, A.
中科院分区：
文献类型：
--
作者：
Almarestani, L.;Waters, S. M.;Ribeiro-Da-Silva, A.
脊髓板层I（LI）神经元在传递和整合疼痛相关信息中起着重要作用，这些信息被传递到更高的中枢。这些神经元兴奋性的改变影响慢性疼痛的发展，神经激肽1受体（NK-1 r）的表达被认为在这种变化中起主要作用。NK-1 r的新表达可能是新的LI神经元群体中过度兴奋的基础。LI投射神经元在形态学上可分为梭形、锥体和多极细胞，它们的功能特性不同，锥体型是非伤害性的。与此一致，我们已经表明，spinoparabrachial锥体神经元很少表达NK-1 r，与其他两种细胞类型相比。在这项研究中，我们调查了大鼠NK-1 R表达的长期变化，脊髓臂旁LI神经元后，单侧注射在后爪足底表面的完全弗氏佐剂（CFA）。将霍乱毒素亚单位B（CT B）注入臂旁核。我们的研究结果表明，同侧，锥体神经元很少免疫反应的NK-1 R在盐水注射和CFA注射大鼠，长达10天后CFA。然而，相当数量的锥体细胞免疫反应NK-1 r在15，21和30天后CFA。我们的数据提高的可能性，这需要通过电生理学证实，大多数LI投射神经元的锥体类型可能是nonhociceptive在幼稚动物，但可能成为伤害性关节炎的发展。神经学比较杂志514：284-295，2009. (C)2009 Wiley-Liss，Inc.
Spinal lamina I (LI) neurons play a major role in the transmission and integration of pain-related information that is relayed to higher centers. Alterations in the excitability of these neurons influence chronic pain development, and expression of the neurokinin 1 receptor (NK-1r) is thought to play a major role in such changes. Novel expression of NK-1r may underlie hyperexcitability in new populations of LI neurons. LI projection neurons can be classified morphologically into fusiform, pyramidal, and multipolar cells, differing in their functional properties, with the pyramidal type being nonnociceptive. In agreement with this, we have shown that spinoparabrachial pyramidal neurons seldom express NK-1r, in contrast with the other two cell types. In this study we investigated in the rat the long-term changes in NK-1r expression by spinoparabrachial LI neurons following the unilateral injection in the hindpaw plantar surface of complete Freund's adjuvant (CFA). Cholera toxin subunit B (CTb) was injected unilaterally into the parabrachial nucleus. Our results revealed that, ipsilaterally, pyramidal neurons were seldom immunoreactive for NK-1r both in saline-injected and in CFA-injected rats, up to 10 days post-CFA. However, a considerable number of pyramidal cells were immunoreactive for NK-1r at 15, 21, and 30 days post-CFA. Our data raise the possibility-which needs to be confirmed by electrophysiology-that most LI projection neurons of the pyramidal type are likely nonnociceptive in naive animals but might become nociceptive following the development of arthritis. J. Comp. Neurol. 514:284-295, 2009. (C) 2009 Wiley-Liss, Inc.