Polymer Scaffolds with Preferential Parallel Grooves Enhance Nerve Regeneration
Polymer Scaffolds with Preferential Parallel Grooves Enhance Nerve Regeneration
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DOI：
10.1089/ten.tea.2014.0266
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发表时间：
2015-03-01
影响因子：
4.1
通讯作者：
Reid, Adam J.
中科院分区：
文献类型：
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作者：
Mobasseri, Atefeh;Faroni, Alessandro;Reid, Adam J.
我们对聚己内酯(PCL)和聚乳酸(PLA)共混膜的表面形貌进行了修饰，以促进细胞增殖和引导周围神经的再生。用图案化硅模板、斜壁(SL)、V形(V)和正方形(SQ)制备了具有不同形状凹槽的薄膜，并与具有微坑的非凹槽表面进行了比较。采用成体脂肪干细胞分化为雪旺细胞样细胞，对溶剂铸膜进行体外实验。细胞在凹槽表面的附着、增殖和定向都得到了改善，其中SL凹槽的效果最好。我们提供了SD大鼠坐骨神经损伤的体内数据，间隙为10 mm，评估了3周后通过管腔内沟槽(SL、V和SQ)和不同壁厚(70、100、120和210微米)改良的聚合物神经导管的神经再生。SL沟神经导管较其他形态沟槽有明显的改善，而增加导管壁厚对再生神经的生物反应无积极作用。此外，在大鼠坐骨神经10 mm间隙模型上，比较了壁厚为70µm的首选SL槽管(C)与目前临床金标准的自体神经移植(Ag)。术后3周，用再生神经纤维桥接两组间的所有神经间隙。16周时，自体移植(Ag)组和导管移植(C)组再生轴突的特征相似。末端器官对肌肉重量、肌电和皮肤神经再支配的评估在两组之间也是相似的。与自体神经移植相比较的实验结果表明，具有内腔微结构沟槽的PCL/PLA管道可作为周围神经修复的一种潜在的替代治疗方法。
We have modified the surface topography of poly e-caprolactone (PCL) and polylactic acid (PLA) blended films to improve cell proliferation and to guide the regeneration of peripheral nerves. Films with differing shaped grooves were made using patterned silicon templates, sloped walls (SL), V-shaped (V), and square-shaped (SQ), and compared with nongrooved surfaces with micropits. The solvent cast films were tested in vitro using adult adipose-derived stem cells differentiated to Schwann cell-like cells. Cell attachment, proliferation, and cell orientation were all improved on the grooved surfaces, with SL grooves giving the best results. We present in vivo data on Sprague-Dawley rat sciatic nerve injury with a 10-mm gap, evaluating nerve regeneration at 3 weeks across a polymer nerve conduit modified with intraluminal grooves (SL, V, and SQ) and differing wall thicknesses (70, 100, 120, and 210 mu m). The SL-grooved nerve conduit showed a significant improvement over the other topographical-shaped grooves, while increasing the conduit wall thickness saw no positive effect on the biological response of the regenerating nerve. Furthermore, the preferred SL-grooved conduit (C) with 70 mu m wall thickness was compared with the current clinical gold standard of autologous nerve graft (Ag) in the rat 10-mm sciatic nerve gap model. At 3 weeks postsurgery, all nerve gaps across both groups were bridged with regenerated nerve fibers. At 16 weeks, features of regenerated axons were comparable between the autograft (Ag) and conduit (C) groups. End organ assessments of muscle weight, electromyography, and skin reinnervation were also similar between the groups. The comparable experimental outcome between conduit and autograft, suggests that the PCL/PLA conduit with inner lumen microstructured grooves could be used as a potential alternative treatment for peripheral nerve repair.