KLF4 regulates the expression of interleukin-10 in RAW264.7 macrophages
KLF4 regulates the expression of interleukin-10 in RAW264.7 macrophages
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KLF4 调节 RAW264.7 巨噬细胞中白细胞介素 10 的表达。
DOI：
10.1016/j.bbrc.2007.07.157
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发表时间：
2007-10-26
影响因子：
3.1
通讯作者：
Xiao, Xianzhong
中科院分区：
文献类型：
--
作者：
Liu, Junwen;Zhang, Huali;Xiao, Xianzhong
krtippel样因子4 （KLF4）是一种进化保守的含锌指转录因子。本研究采用大肠杆菌脂多糖（Escherichia coli lipopolaccharide， LPS）处理RAW264.7小鼠巨噬细胞，测定KLF4的表达。将KLF4全长cDNA或反义寡核苷酸转染RAW264.7巨噬细胞，采用RT-PCR和ELISA分析IL-10的表达和释放情况。通过荧光素酶报告基因和电泳迁移转移实验检测KLF4对IL-10启动子的转录和DNA结合活性。结果表明，LPS处理RAW264.7巨噬细胞导致KLF4蛋白水平呈剂量依赖性和时间依赖性升高；KLF4过表达使IL-10表达升高，而KLF4抑制使IL-10表达降低。KLF4的过表达促进了KLF4对IL-10启动子的转录和DNA结合活性。这些结果表明KLF4在调节IL-10的表达中起重要作用。(c) 2007爱思唯尔公司版权所有。
Krtippel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor. In the current study, RAW264.7 murine macrophages were treated with Escherichia coli lipopolysaccharide (LPS) to determine the expression of KLF4. A full-length cDNA or antisense oligonucleotides of KLF4 was transfected into RAW264.7 macrophages, and the expression and release of IL-10 were analyzed by RT-PCR and ELISA. The transcription and DNA binding activities of KLF4 to the IL-10 promoter were detected by the luciferase reporter and electrophoretic mobility shift assays. The results showed that treatment of RAW264.7 macrophages with LPS resulted in a dose- and time-dependent increase in KLF4 protein levels; KLF4 overexpression increased the expression of IL-10, while KLF4 inhibition decreased the expression of IL-10. The overexpression of KLF4 promoted the transcription and DNA binding activities of KLF4 to the IL-10 promoter. These results indicate that KLF4 plays an important role in regulating the expression of IL-10. (c) 2007 Elsevier Inc. All rights reserved.