DNA Transposition at Work.
DNA Transposition at Work.
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DOI：
10.1021/acs.chemrev.6b00003
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发表时间：
2016-10-26
期刊：
影响因子：
62.1
通讯作者：
Dyda F
中科院分区：
文献类型：
--
作者：
Hickman AB;Dyda F
DNA转座子是能够从一个基因组位置移动到另一个基因组位置的定义的DNA片段。运动由一种或多种称为转座酶的蛋白质促进，通常由移动的元件本身编码。在这里，我们首先提供了一个概述的分类，这种移动的元素在各种生物体。从机制的角度来看，我们集中在一个特定的DNA转座子组，编码一个转座酶与DD（E/D）催化结构域，是拓扑相似的RNA酶H。对于这些，一些转座体（转座酶-核酸复合物）的三维结构是可用的，我们用这些来描述其机制的基础。DD（E/D）组除了是所有DNA转座酶中最大和最常见的之外，其成员还被用于各种各样的基因组应用。因此，本文的第二个重点是提供转座子应用的非详尽概述。尽管在过去十年中出现了几种基于非转座子的定点基因组修饰方法，但当不寻求整合特异性时，基于转座子的应用是高度相关的。事实上，对于许多应用来说，几乎完美的随机性和高频率的整合使得基于转座子的方法不可或缺。
DNA transposons are defined segments of DNA that are able to move from one genomic location to another. Movement is facilitated by one or more proteins, called the transposase, typically encoded by the mobile element itself. Here, we first provide an overview of the classification of such mobile elements in a variety of organisms. From a mechanistic perspective, we have focused on one particular group of DNA transposons that encode a transposase with a DD(E/D) catalytic domain that is topologically similar to RNase H. For these, a number of three-dimensional structures of transpososomes (transposase–nucleic acid complexes) are available, and we use these to describe the basics of their mechanisms. The DD(E/D) group, in addition to being the largest and most common among all DNA transposases, is the one whose members have been used for a wide variety of genomic applications. Therefore, a second focus of the article is to provide a nonexhaustive overview of transposon applications. Although several non-transposon-based approaches to site-directed genome modifications have emerged in the past decade, transposon-based applications are highly relevant when integration specificity is not sought. In fact, for many applications, the almost-perfect randomness and high frequency of integration make transposon-based approaches indispensable.
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影响因子：
5.6
作者：
Biery, MC;Lopata, M;Craig, NL
通讯作者：
Craig, NL
影响因子：
13.8
作者：
Aravind, L
通讯作者：
Aravind, L
影响因子：
3.7
作者：
Lane MA;Kimber M;Khokha MK
通讯作者：
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DOI：
10.1002/lt.24122
发表时间：
2015-06
期刊：
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1007/978-1-61779-603-6_13
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Ammar, Ismahen;Izsvak, Zsuzsanna;Ivics, Zoltan
通讯作者：
Ivics, Zoltan