Taxonomic study of Marinomonas strains isolated from the seagrass Posidonia oceanica, with descriptions of Marinomonas balearica sp nov and Marinomonas pollencensis sp nov.
Taxonomic study of Marinomonas strains isolated from the seagrass Posidonia oceanica, with descriptions of Marinomonas balearica sp nov and Marinomonas pollencensis sp nov.
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DOI：
10.1099/ijs.0.008607-0
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Sanchez-Amat, Antonio
中科院分区：
文献类型：
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作者：
Espinosa, Elena;Marco-Noales, Ester;Sanchez-Amat, Antonio
从与地中海海草Posidonia Ocean anica相关的可培养微生物群中分离到DNA G+C含量低于50mol%的新型需氧革兰氏阴性细菌。16S rRNA基因序列分析表明，它们属于Marinomonas属。菌株Ivia-Po-186是海洋单胞菌的一株，与模式菌株MMB-1(T)的16S rRNA基因序列具有99.77%的相似性，并具有所研究的所有表型特征。这是对形成海洋植物微生物区系一部分的本种的第一次描述。第二个菌株命名为Ivia-Po-101(T)，根据系统发育研究，它与地中海分支杆菌有密切的亲缘关系。然而，它在黑色素合成和酪氨酸酶、漆酶和抗菌活性等特性上存在差异。此外，菌株Ivia-Po-101(T)具有营养缺陷性，不能利用醋酸盐。Ivia-Po-101(T)与地中海分枝杆菌MMB-1(T)的16S rRNA基因序列同源性为97.86%，但DNA-DNA同源性仅为10.3%。在这些数据的基础上，菌株Ivia-Po-101(T)被认为是Marinomonas属的一个新种，命名为Marinomonas balearica sp.11月是提议的。模式菌株为Ivia-Po-101(T)(=CECT7378(T)=NCIMB 14432(T))。第三个新菌株，Ivia-Po-185(T)，在系统发育上与所有已知的Marinomonas物种相距甚远。与模式菌株的16S rRNA基因序列相似性最高(97.4%)，但DNA-DNA同源性仅为14.5%。该菌株在Marinomonas属中的一个不同的化学分类标志是脂肪酸C-17：0 clo的存在。因此，菌株Ivia-Po-185(T)被认为是该属的第二个新种，命名为Marinomonas pollencens sp.11月是提议的。模式菌株为Ivia-Po-185(T)(=CECT7375(T)=NCIMB 14435(T))。在对这两个新种的描述的基础上，对Marinomonas属进行了订正描述，并对原描述后的其他Marinomonas种进行了描述。
Novel aerobic, Gram-negative bacteria with DNA G + C contents below 50 mol% were isolated from the culturable microbiota associated with the Mediterranean seagrass Posidonia oceanica. 16S rRNA gene sequence analyses revealed that they belong to the genus Marinomonas. Strain IVIA-Po-186 is a strain of the species Marinomonas mediterranea, showing 99.77% 16S rRNA gene sequence similarity with the type strain, MMB-1(T), and sharing all phenotypic characteristics studied. This is the first description of this species forming part of the microbiota of a marine plant. A second strain, designated IVIA-Po-101(T), was closely related to M. mediterranea based on phylogenetic studies. However, it differed in characteristics such as melanin synthesis and tyrosinase, laccase and antimicrobial activities. In addition, strain IVIA-Po-101(T) was auxotrophic and unable to use acetate. IVIA-Po-101(T) shared 97.86% 16S rRNA gene sequence similarity with M. mediterranea MMB-1(T), but the level of DNA-DNA relatedness between the two strains was only 10.3%. On the basis of these data, strain IVIA-Po-101(T) is considered to represent a novel species of the genus Marinomonas, for which the name Marinomonas balearica sp. nov. is proposed. The type strain is IVIA-Po-101(T) (=CECT 7378(T) =NCIMB 14432(T)). A third novel strain, IVIA-Po-185(T), was phylogenetically distant from all recognized Marinomonas species. It shared the highest 16S rRNA gene sequence similarity (97.4%) with the type strain of Marinomonas pontica, but the level of DNA-DNA relatedness between the two strains was only 14.5%. A differential chemotaxonomic marker of this strain in the genus Marinomonas is the presence of the fatty acid C-17:0 cyclo. Strain IVIA-Po-185(T) is thus considered to represent a second novel species of the genus, for which the name Marinomonas pollencensis sp. nov. is proposed. The type strain is IVIA-Po-185(T) (=CECT 7375(T) =NCIMB 14435(T)). An emended description of the genus Marinomonas is given based on the description of these two novel species, as well as other Marinomonas species described after the original description of the genus.