Achromosomal Division of Early Starfish Embryos Cultured in the Presence of Actinomycin D
Achromosomal Division of Early Starfish Embryos Cultured in the Presence of Actinomycin D
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放线菌素 D 存在下培养的早期海星胚胎的无染色体分裂
DOI：
10.1111/j.1440-169x.1991.00193.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
N. Itoh
中科院分区：
文献类型：
--
作者：
S. Ikegami;Y. Ozaki;Y. Ooe;N. Itoh
研究了海星Asterina pectinifera的胚胎在放线菌素D（一种最广泛使用的RNA合成抑制剂）存在下经历胚胎发育早期事件的能力。在25 μg ml - 1放线菌素D的作用下，受精卵继续分裂8或9次，但发育迟缓。放线菌素D处理胚胎的卵裂球染色质在32细胞期发生崩解，细胞核在后期无法检测到。在32细胞阶段之前，放线菌素D的存在不影响RNA合成，但DNA合成受到严重抑制。染色体分裂停止和胚胎开始死亡的阶段对应于囊胚开始前的时期，这表明细胞核和染色体的存在是该物种在512个细胞阶段之后囊胚形成和发育的先决条件。
Embryos of the starfish Asterina pectinifera were examined for their ability to undergo the early events of embryonic development in the presence of actinomycin D, a most widely used inhibitor of RNA synthesis. Fertilized eggs continued to divide eight or nine times in the presence of 25 μg ml−1actinomycin D, although delay of development was observed. Chromatin disintegrated in the blastomeres of actinomycin D‐treated embryos specifically at the 32‐cell stage and the nucleus was undetectable at later stages. Before the 32‐cell stage, RNA synthesis was not affected by the presence of actinomycin D whereas DNA synthesis was severely inhibited. The stage when achromosomal divisions cease and embryos begin to die corresponds to the period just before onset of blastulation, suggesting that the presence of the nucleus and chromosomes is a prerequisite for blastula formation and development beyond the 512‐cell stage in this species.