Magnesium-based bone implants: Immunohistochemical analysis of peri-implant osteogenesis by evaluation of osteopontin and osteocalcin expression
Magnesium-based bone implants: Immunohistochemical analysis of peri-implant osteogenesis by evaluation of osteopontin and osteocalcin expression
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DOI：
10.1002/jbm.a.34828
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发表时间：
2014-05-01
影响因子：
4.9
通讯作者：
Reifenrath, J.
中科院分区：
文献类型：
--
作者：
Bondarenko, A.;Angrisani, N.;Reifenrath, J.
骨桥蛋白（osteopontin，OPN）和骨钙素（osteocalcin，OC）等骨蛋白的功能已被最新研究发现。将镁合金（MgCa0.8，LAE 442，ZEK 100，LANd 442）和钛合金（TiAl 6V 4）圆柱形钉植入46只家兔胫骨髓内。根据植入持续时间（3个月和6个月）对每组进行分组。将种植体附近的骨样本脱钙，并使用OC和OPN抗体进行常规组织学和免疫组织化学方案处理。OC主要分布于密质骨基质中，而在类骨质和骨细胞中很少见到。骨桥蛋白在细胞内和类骨质中均有表达。3个月后，钛组的两种标记物水平最高，其次是LAE 442组。与LAE 442和TiAl 6V 4相比，其他镁合金在6个月后显示出增加的OC水平。与对照组相比，OP和OC水平较低与术后早期种植体持续降解和骨-种植体界面不稳定有关。6个月后LAE 442和TiAl 6V 4组中标记物表达的降低可能表明骨-种植体界面的稳定和种植体周围新骨生成的完成。OC和OPN表达随植入时间的下降特征，以及其在术后晚期的最低水平，表明LAE 442具有更适当的生物相容性，因此似乎是用于骨科应用的最优选的测试材料。(c)2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：102A：1449-1457，2014.
The functions of some bone proteins, as osteopontin (OPN) and osteocalcin (OC), have been discovered by the latest studies. This fact suggests the possibility of their immunodetection to characterize peri-implant osteogenesis and implant impact on it. Cylindrical pins of Mg alloys (MgCa0.8, LAE442, ZEK100, LANd442) and titanium alloy (TiAl6V4) were implanted into the tibial medullae of 46 rabbits. Each group was divided regarding to implant duration (3 and 6 months). Bone samples adjacent to the implants were decalcified and treated with routine histological and immunohistochemical protocols using OC and OPN-antibodies. OC was detected in matrix of compact bone, but very rarely in osteoid and bone cells. OPN was detected intracellularly and in osteoid. After 3 months, the highest level of both markers was found in titanium group, followed by LAE442-group. In contrast to LAE442 and TiAl6V4, the other Mg alloys showed increasing levels of OC after 6 months. Lower levels of OP and OC compared to the control group are related to the continuous implant degradation and instability of bone-implant interface in early post-surgical period. Reduced marker's expression in LAE442 and TiAl6V4 groups after 6 months may indicate stabilization of bone-implant interface and completion of peri-implant neo-osteogenesis. Declining characters of OC and OPN expression over the implantation time, as well as their lowest levels in late post-surgical term, suggest a more appropriate biocompatibility of LAE442, which therefore seems to be the most preferable of the tested materials for the use in orthopaedic applications. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1449-1457, 2014.