pliced variants of period mRNA in the Japanese honeybee Apis cerana japomca
pliced variants of period mRNA in the Japanese honeybee Apis cerana japomca
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日本蜜蜂 Apis cerana japomca 中 period mRNA 的剪接变体
DOI：
10.1080/09291016.2010.537447
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发表时间：
2012
影响因子：
1.1
通讯作者：
Shim izu I
中科院分区：
文献类型：
--
作者：
Minamoto T;Fuchikawa T;Shim izu I
我们报道了蜜蜂period（per）基因的两种mRNA变体α和β的表达。在其他昆虫中没有发现。日本蜜蜂（Apis cerana japonica）前基因的基因组DNA序列表明，变异体是由内含子的选择性剪接产生的。脑中的总mRNA在黑暗期增加，在光照下减少，而肌肉中的mRNA则表现出相反的相位振荡。复眼和中肠中未观察到permRNA的明显节律性表达。在12：12 h光暗（LD）条件下，研究了在Zeitgeber时间（ZT）6和18（分别为白天和夜晚）时permRNA α和β变体的表达比例。在黑暗中，两个器官中的per α变体的表达增加，而β变体的表达在器官之间显示出相反的模式。在光照期肌肉中较高的totalpermRNA表达是由于β变体的增加。这种表达模式表明α和β变体之间存在功能差异。此外，我们还发现其他种类的Hyplettera也具有这种α和β变体的同源物，这表明它们在该目昆虫中具有特定的功能。
We report the expression of two mRNA variants, α and β, of theperiod(per) gene in honeybees. These have not been found in other insects. The genomic DNA sequence of thepergene in the Japanese honeybee (Apis cerana japonica) showed that the variants are produced by alternative splicing of an intron. TotalpermRNA in the brain increased in the dark period and decreased in the light, whereas mRNA of the muscle showed an opposite phase oscillation. No significant rhythmic expression ofpermRNA was observed in the compound eye and midgut. The expression ratio of the α and β variants ofpermRNA was investigated at zeitgeber times (ZTs) 6 and 18 (the middle of the day and night, respectively) under 12:12 h light–dark (LD) conditions. Theperα variant increased in the dark in both organs, whereas expression of the β variant showed an opposite pattern between the organs. The higher totalpermRNA expression in muscle in the light period was due to an increase in the β variant. Such expression patterns suggest that there is a functional differentiation between the α and β variants. Furthermore, we found that other species of Hymenoptera possess homologs of theperα and β variants, suggesting that they have a specific function in this order of insects.