Selection of kidney cell types in primary glomerular explant outgrowths by in vitro culture conditions.
Selection of kidney cell types in primary glomerular explant outgrowths by in vitro culture conditions.
复制标题
通过体外培养条件选择原代肾小球外植体中肾细胞类型。
作者：
T. Oberley;A. H. Yang;J. Gould
成年豚鼠肾小球在血清或化学成分确定的培养基中体外生长。将肾小球直接接种到塑料烧瓶中，或接种到已经用PF-HR-9小鼠畸胎瘤内胚层细胞系产生的细胞外基质包被的塑料烧瓶中。培养基的组成和培养基质的性质都影响整个肾小球的附着和培养中产生的细胞类型。定量研究证明了不同培养条件对细胞类型的选择。三个集落类型，每一个独特的细胞类型组成，可以确定的形态特征。这些集落类型的细胞构成的上皮性质，但它们被确定为不同的上皮类型的组织化学和超微结构标准。以往的研究表明，一种上皮细胞类型来自肾小球内脏上皮细胞。这项研究表明，这种细胞类型可以选择性地生长在塑料上的限定培养基中。第二种细胞类型显示出肾小管上皮细胞的几个特征，包括过氧化氢酶、细胞表面微绒毛和纤毛的组织化学染色，以及长时间培养后形成半囊和类似肾小管的结构。为了证明“肾小球衍生的”肾小管细胞确实是肾小管上皮细胞，我们分离纯化的肾皮质小管（纯度大于99%），并将其培养在无血清化学成分确定的培养基中的HR-9基质上。所得到的产物具有与肾小球衍生的肾小管细胞相同的形态学特性。这似乎是可能的小管碎片连接到少数肾小球的来源，这些肾小球衍生的肾小管细胞。两种上皮细胞类型都不能在塑料上传代培养，但都可以在HR-9基质上传代。第三种细胞类型，纺锤形细胞，很容易在塑料和HR-9基质上繁殖。这种细胞类型的起源尚不清楚。我们的研究结果表明，在体外培养条件对肾细胞类型的选择，生长和分化的重要影响。
Adult guinea pig glomeruli were grown in vitro either in serum or in a chemically defined medium. Glomeruli were plated either directly into plastic flasks or into plastic flasks that had been coated with the extracellular matrix produced by the PF-HR-9 mouse teratocarcinoma endodermal cell line. Both the composition of the medium and the nature of the culture substrate affected whole glomerular attachment and the type of cells produced in culture. Quantitative studies demonstrated selection of cell types by different culture conditions. Three colony types, each composed of distinctive cell types, could be identified by morphological features. The cells constituting two of these colony types were epithelial in nature, but they were identified as different epithelial types by both histochemical and ultrastructural criteria. Previous studies suggested that one epithelial cell type was derived from the glomerular visceral epithelial cell. This study demonstrates that this cell type could be selectively grown in defined medium on plastic. A second cell type showed several features of renal tubular epithelial cells, including histochemical staining for catalase, cell surface microvilli and cilia, and formation of hemicysts and structures that resembled tubules after prolonged periods in culture. To demonstrate that the 'glomerulus-derived' tubular cells were indeed tubular epithelium, we isolated purified renal cortical tubules (greater than 99% pure) and cultured them on the HR-9 matrix in a serum-free chemically defined medium. The resultant outgrowths had morphological properties identical to those of the glomerulus-derived tubular cells. It seems likely that small tubular fragments attached to a minority of the glomeruli are the source of these glomerulus-derived tubular cells. Neither epithelial cell type could be subcultured on plastic, but both could be passaged on the HR-9 matrix. A third cell type, the spindle-shaped cell, was easily propagated on both plastic and the HR-9 matrix. The origin of this cell type is not clear. Our results demonstrate the important effect of culture conditions on the selection, growth and differentiation of kidney cell types in vitro.
纤连蛋白二聚体和多聚体形式对原代肾小球细胞粘附和生长的影响。
影响因子：
3.7
作者：
Oberley,TD;Murphy-Ullrich,JE;Albrecht,RM;Mosher,DF
通讯作者：
Mosher,DF
研究糖蛋白 GP-2 在体内肾小球内和体外培养的肾细胞株中的定位。
DOI：
10.1177/29.11.7033360
发表时间：
1981
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Oberley,TD;Chung,AE;Murphy-Ullrich,JE;Mosher,DF
通讯作者：
Mosher,DF