Guided bone regeneration membrane made of polycaprolactone/calcium carbonate composite nano-fibers
Guided bone regeneration membrane made of polycaprolactone/calcium carbonate composite nano-fibers
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DOI：
10.1016/j.biomaterials.2004.09.014
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发表时间：
2005-07-01
期刊：
影响因子：
14
通讯作者：
Ramakrishna, S
中科院分区：
文献类型：
--
作者：
Fujihara, K;Kotaki, M;Ramakrishna, S
在这项研究中，新型的引导骨再生（GBR）膜制备的聚己内酯（PCL）/碳酸钙（CaCO 3）复合纳米纤维与两个不同的PCL碳酸钙（PCL：75：25重量%和25：75重量%）。采用静电纺丝法成功制备了复合纳米纤维，并通过能谱分析证实了纤维表面存在CaCO 3纳米粒子。为了实现GBR膜的机械稳定性，将复合纳米纤维纺在具有高拉伸强度的PCL纳米纤维膜上，即，该膜由功能层（PCL/CaCO 3）和机械支撑层（PCL）两层组成。制备两种不同的GBR膜，即，GBR膜（A）= PCL：CaCO 3 = 75：25重量% + PCL，GBR膜（B）= PCL：CaCO 3 = 25：75重量% + PCL。通过MTS测定和扫描电镜（SEM）观察，探讨成骨细胞在GBR膜（A）和（B）上的附着和增殖情况。结果，GBR膜（A）和组织培养聚苯乙烯（TCPS）的吸光度强度在5天接种时间期间增加。相反，尽管GBR膜（B）的吸光度强度也增加，但其值低于膜（A）。扫描电镜观察显示，成骨细胞在GBR膜（A）和（B）上的附着方式无显著差异。由于PCL/CaCO 3复合纳米纤维具有良好的细胞附着方式，因此有可能将其应用于GBR膜。(C)2004爱思唯尔有限公司保留所有权利。
In this study, new type of guided bone regeneration (GBR) membranes were fabricated by polycaprolactone (PCL)/CaCO3 composite nano-fibers with two different PCL to calcium carbonate (CaCO3) ratios (PCL:CaCO3 75:25 wt% and 25:75 wt%). The composite nano-fibers were successfully fabricated by electrospinning method and CaCO3 nano-particles on the surface of nanofibers were confirmed by energy disperse X-ray (EDX) analysis. In order to achieve mechanical stability of GBR membranes, composite nano-fibers were spun on PCL nano-fibrous membranes which has high tensile strength, i.e., the membranes consist of two layers of functional layer (PCL/CaCO3) and mechanical support layer (PCL). Two different GBR membranes were prepared, i.e., GBR membrane (A) = PCL:CaCO3 = 75:25 wt% + PCL, GBR membrane (B) = PCL:CaCO3 = 25:75 wt% + PCL. Osteoblast attachment and proliferation of GBR membrane (A) and (B) were discussed by MTS assay and scanning electron microscope (SEM) observation. As a result, absorbance intensity of GBR membrane (A) and tissue culture polystyrene (TCPS) increased during 5 days seeding time. In contrast, although absorbance intensity of GBR membrane (B) also increased, its value was lower than membrane (A). SEM observation showed that no significant difference in osteoblast attachment manner was seen on GBR membrane (A) and (B). Because of good cell attachment manner, there is a potential to utilize PCL/CaCO3 composite nano-fibers to GBR membranes. (C) 2004 Elsevier Ltd. All rights reserved.