Antioxidant responses of Chilo suppressalis (Lepidoptera: Pyralidae) larvae exposed to thermal stress
Antioxidant responses of Chilo suppressalis (Lepidoptera: Pyralidae) larvae exposed to thermal stress
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DOI：
10.1016/j.jtherbio.2011.04.003
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发表时间：
2011-07-01
影响因子：
2.7
通讯作者：
Qiang, Chengkui
中科院分区：
文献类型：
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作者：
Cui, Yadong;Du, Yuzhou;Qiang, Chengkui
高温引起昆虫的多种生理应激反应，包括增加活性氧（ROS）的产生，这可能导致氧化损伤。研究了高温胁迫对二化螟（Chilo suppressalis）幼虫血细胞活性氧（ROS）产生、热休克蛋白70（Hsp70）mRNA和蛋白表达、抗氧化酶（SOD、CAT）活性以及细胞凋亡的影响。结果表明，热胁迫显著提高了红球藻体内活性氧水平和抗氧化酶活性。二化螟幼虫实时荧光定量PCR结果显示热胁迫诱导了hsp70基因的表达。流式细胞仪检测结果显示，Hsp70在蛋白水平和mRNA水平的表达谱基本一致。在36 ℃时，Hsp70在mRNA和蛋白水平的表达量均达到最大值。暴露于测试的温度没有引起任何显着的变化，在幼虫血细胞的凋亡率。这些结果表明，热应激导致氧化应激，抗氧化酶和热休克蛋白70在减少氧化损伤中起重要作用。二化螟幼虫(C)2011爱思唯尔有限公司保留所有权利。
High temperatures cause a variety of physiological stress responses in insects, including increased generation of reactive oxygen species (ROS), which can cause oxidative damage. This study investigated the effects of thermal stress on ROS generation, the expression of heat shock protein 70 (Hsp70) at the mRNA and protein levels, the activity of antioxidant enzymes (SOD, CAT), and apoptosis in hemocytes of Chilo suppressalis larvae. Results indicated that thermal stress significantly elevated the level of ROS and antioxidant enzyme activity in C. suppressalis larvae. Real-time quantitative PCR showed that hsp70 gene expression was induced by heat stress. Flow cytometric results revealed that the expression profile of Hsp70 at the protein level was in agreement with that at the mRNA level. The expression of Hsp70 at both the mRNA and protein levels reached a maximum at 36 degrees C in larval hemocytes. Exposure to tested temperatures did not cause any significant change in the rate of apoptosis in larval hemocytes. These results suggest that thermal stress leads to oxidative stress and that antioxidant enzymes and the Hsp70 play an important role in reducing oxidative damage in C. suppressalis larvae. (C) 2011 Elsevier Ltd. All rights reserved.