Studies of human diploid fibroblast growth. I. Responses of normal and hypopituitary cells to fibroblast growth factor, insulin, and serum.
Studies of human diploid fibroblast growth. I. Responses of normal and hypopituitary cells to fibroblast growth factor, insulin, and serum.
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人二倍体成纤维细胞生长的研究。
DOI：
10.1210/jcem-43-4-721
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
J. Addison
中科院分区：
文献类型：
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作者：
K. Jones;J. Addison
我们测定了牛胰岛素、成纤维细胞生长因子（FGF）和胎牛血清（FBS）对正常和特发性低分化供体二倍体人成纤维细胞的生长刺激活性。所有这三个因素刺激DNA合成的细胞被血清饥饿。FGF在低至5 ng/ml的浓度下具有活性，在100 ng/ml时具有最大作用。FGF刺激DNA合成的浓度低于胰岛素，也产生了更大的最大响应。只有血清才能支持细胞分裂和生长，但当FGF加入含血清的培养基中时，它加速了这种生长速率。氢化可的松，放线菌素D，放线菌酮抑制FGF的刺激。正常和低增殖供体的成纤维细胞之间无显著差异。
We have assayed the growth stimulating activity of bovine insulin, fibroblast growth factor (FGF), and fetal bovine serum (FBS) in diploid human fibroblasts from normal and idiopathic hypopituitary donors. All three factors stimulated DNA synthesis in cells arrested by serum starvation. FGF was active at concentrations as low as 5 ng/ml with maximum effect at 100 ng/ml. FGF stimualted DNA synthesis at lower concentrations than did insulin and also produced a greater maximum response. Only serum was capable of supporting cell division and growth, but FGF accellerated this growth rate when it was added to serum-containing medium. Hydrocortisone, actinomycin D, and cycloheximide inhibit FGF stimulation. There was no significant difference between fibroblasts from normal and hypopituitary donors.