CrRLK1L receptor-like kinases HERCULES RECEPTOR KINASE 1 and ANJEA are female determinants of pollen tube reception
CrRLK1L receptor-like kinases HERCULES RECEPTOR KINASE 1 and ANJEA are female determinants of pollen tube reception
复制标题
CrRLK1L 受体样激酶 HERCULES 受体激酶 1 和 ANJEA 是花粉管接收的女性决定因素
DOI：
10.1101/428854
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Galindo-Trigo S
中科院分区：
文献类型：
--
作者：
Galindo-Trigo S
配子体之间的通讯对被子植物的受精至关重要。多个CrRLK 1 L型受体激酶防止过早的花粉管爆裂，而另一个CrRLK 1 L蛋白，FERONIA（FER），需要在雌配子体中的花粉管爆裂。我们在这里报告的鉴定另外两个CrRLK 1 L同源物，HERK 1和ANJEA（ANJ），这是多余的，以促进助细胞的花粉管爆裂。HERK 1和ANJ定位于未受精胚珠中助细胞的丝状器，并且在herk 1 anj突变体中，大多数胚珠由于花粉管过度生长而保持未受精，这共同表明HERK 1和ANJ充当受精的雌性决定因素。在FER突变体中，助细胞特异性，内膜蛋白NORTIA（NTA）是不重新定位后，花粉管接收，然而，与FER突变体，活性氧水平不受影响的herk 1 ANJ双突变体。ANJ和HERK 1都与FER及其在植物中的辅助受体LORELEI（LRE）相关。总之，我们的数据表明，HERK 1和ANJ与FER一起介导植物受精过程中的雌-雄配子体相互作用。
Communication between the gametophytes is vital for angiosperm fertilisation. Multiple CrRLK1L-type receptor kinases prevent premature pollen tube burst, while another CrRLK1L protein, FERONIA (FER), is required for pollen tube burst in the female gametophyte. We report here the identification of two additional CrRLK1L homologues, HERCULES RECEPTOR KINASE 1 (HERK1) and ANJEA (ANJ), which act redundantly to promote pollen tube burst at the synergid cells. HERK1 and ANJ localise to the filiform apparatus of the synergid cells in unfertilised ovules, and in herk1 anj mutants a majority of ovules remain unfertilised due to pollen tube overgrowth, together indicating that HERK1 and ANJ act as female determinants for fertilisation. As in fer mutants, the synergid cell-specific, endomembrane protein NORTIA (NTA) is not relocalised after pollen tube reception; however, unlike fer mutants, reactive oxygen species levels are unaffected in herk1 anj double mutants. Both ANJ and HERK1 associate with FER and its proposed co-receptor LORELEI (LRE) in planta. Together, our data indicate that HERK1 and ANJ act with FER to mediate female-male gametophyte interactions during plant fertilisation.