MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1.
MiR-29c inhibits cell growth, invasion, and migration of pancreatic cancer by targeting ITGB1.
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DOI：
10.2147/ott.s92758
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发表时间：
2016
影响因子：
4
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Lu Y;Hu J;Sun W;Li S;Deng S;Li M
MiR-29 c在许多癌症中经常失调;然而，miR-29 c在胰腺癌（PC）中的作用及其潜在机制仍然知之甚少。在这项研究中，我们研究了miR-29 c在PC中的作用。使用定量实时聚合酶链反应，我们证明了miR-29 c在临床PC组织和细胞系中频繁下调。过表达miR-29 c可显着抑制PC细胞的体外增殖、迁移和侵袭，这表明miR-29 c在PC细胞中具有肿瘤抑制因子的作用。进一步分析表明，ITGB 1是miR-29 c的功能靶基因之一，ITGB 1的敲低可抑制PC细胞的增殖、迁移和侵袭，这与过表达miR-29 c的作用相似。综上所述，我们的结果强调了miR-29 c-ITGB 1相互作用在PC发生和进展中的重要性。
MiR-29c is frequently dysregulated in many cancers; however, the roles of miR-29c in pancreatic cancer (PC) and underlying mechanisms remain poorly understood. In this study, we investigated the role of miR-29c in PC. Using quantitative real-time polymerase chain reaction, we demonstrated that miR-29c was frequently downregulated in clinical PC tissues and cell lines. Overexpression of miR-29c significantly inhibited the proliferation, migration, and invasion of PC cells in vitro, which demonstrated that miR-29c acts as a tumor suppressor in PC cells. Further analysis revealed that ITGB1 is one of the functional target genes of miR-29c, and knockdown of ITGB1 inhibited the proliferation, migration, and invasion of PC cells, which was similar to the effects of overexpression of miR-29c. Taken together, our results highlight the significance of miR-29c–ITGB1 interaction in the development and progression of PC.