Expression pattern of type 3 adenylyl cyclase in rodent dorsal root ganglion and its primary afferent terminals
Expression pattern of type 3 adenylyl cyclase in rodent dorsal root ganglion and its primary afferent terminals
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啮齿动物背根神经节及其初级传入末梢3型腺苷酸环化酶的表达模式
DOI：
10.1016/j.neulet.2018.10.049
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发表时间：
2019-01-23
影响因子：
2.5
通讯作者：
Zhang Yu-Qiu
中科院分区：
文献类型：
--
作者：
Hu Man-Li;Zhang Wen-Wen;Zhang Yu-Qiu
环磷酸腺苷（cAMP）是研究最多的第二信使之一，受腺苷酸环化酶（AC）家族的调节。3型腺苷酸环化酶（Type 3 adenylyl cyclase，AC 3）是腺苷酸环化酶的一个亚型，广泛表达于嗅觉和中枢神经系统的纤毛中，在嗅觉、发育等生理功能中发挥重要作用。然而，AC 3在背根神经节（DRG）中的表达尚未报道。本研究采用免疫组织化学方法，发现AC 3免疫反应（IR）主要表达于背根神经节（DRG）中小型神经元的胞质中。双标记显示，大多数AC 3 IR与CGRP（肽能伤害感受器标记物）共定位，很少与NF 200（有髓鞘神经元标记物）或IB 4（非肽能伤害感受器标记物）共定位。此外，AC 3免疫反应在背角浅层，尤其是在CGRP阳性神经元终止的II板层和背侧部也有密集的分布。AC 3在C57/BL 6 J小鼠和Sprague道利大鼠中的表达模式相似。例如，AC 3主要在小到中等大小的DRG神经元的细胞体中表达，并且大多数AC 3 IR也在大鼠中的含CGRP的神经元中表达。综上所述，我们的数据表明，AC 3主要表达在小到中等大小的细胞体和CGRP阳性DRG神经元的中央末梢，这意味着AC 3酶可能在伤害性感受中发挥潜在的作用。
CAMP (Cyclic Adenosine monophosphate), one of the most highly studied second messengers, is regulated by a family of adenylyl cyclase (AC) enzymes. Type 3 adenylyl cyclase (abbreviated as AC3), a subtype of adenylyl cyclase, is reported to be expressed in cilia in the olfactory and central nervous system and plays an important role in many physiological functions such as olfaction, development. However, expression of AC3 in the dorsal root ganglion (DRG) is not reported. In the present study, using immunohistochemical method, we discovered that AC3 immunoreactivity (IR) is predominantly expressed in the cytoplasm of small to medium sized DRG neurons. Double labelling revealed that the majority of AC3 IR are colocalized with CGRP (a peptidergic nociceptor marker), rarely with NF200 (a myelinated neuronal marker) or IB4 (a nonpeptidergic nociceptor marker). Furthermore, dense AC3 IR exists in the superficial dorsal horn, especially in laminaland dorsal part of lamina II, where CGRP-positive DRG neurons terminate. The expression pattern of AC3 is similar between C57/BL6 J mouse and Sprague Dawley rat. For instance, AC3 is primarily expressed in the cell bodies of small to medium sized DRG neurons and the majority of AC3 IR is also in CGRP-containing neurons in rat. Taken together, our data suggest that AC3 is primarily expressed in the small to medium sized cell bodies and central terminals of CGRP-positive DRG neurons, implying AC3 enzyme might potentially function in nociception.