Correlation of T-channel coding gene expression, IT, and the low threshold Ca2+ spike in the thalamus of a rat model of absence epilepsy
Correlation of T-channel coding gene expression, IT, and the low threshold Ca2+ spike in the thalamus of a rat model of absence epilepsy
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DOI：
10.1016/j.mcn.2008.07.012
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发表时间：
2008-10-29
影响因子：
3.5
通讯作者：
Budde, Thomas
中科院分区：
文献类型：
--
作者：
Broicher, Tilman;Kanyshkova, Tatyana;Budde, Thomas
丘脑神经元的T型钙电流依赖性爆发放电被认为参与了失神发作期间观察到的超同步活动。在这里，我们调查的T-通道编码基因（α 1G，-H，-I），T型钙电流的表达之间的相关性，并在三个功能不同的丘脑核（外侧膝状体核，中央外侧核，网状核）在癫痫大鼠模型，WAG/Rij大鼠，和非癫痫对照品系，ACI大鼠T-电流依赖性低阈值钙峰。外侧膝状体核和中央外侧核主要表达α 1G和α 1 I，而丘脑网状核表达α 1H和α 1 I。与ACI相比，WAG/Rij中的表达更高。T型钙电流的性质匹配的预测来自表达模式分析。电流密度在WAG/Rij大鼠的所有核相比，ACI和ITS的大小和最小LTS产生斜率相关，而T型钙离子电流电压依赖性与LTS的起始电位。(C)2008年爱思唯尔公司All rights reserved.
T-type Ca2+ Current-dependent burst firing of thalamic neurons is thought to be involved in the hypersynchronous activity observed during absence seizures. Here we investigate the correlation between the expression of T-channel coding genes ((alpha 1G, -H, -I), T-type Ca2+ current, and the T-current-dependent low threshold Ca2+ spike in three functionally distinct thalamic nuclei (lateral geniculate nucleus; centrolateral nucleus; reticular nucleus) in a rat model of absence epilepsy, the WAG/Rij rats, and a non-epileptic control strain, the ACI rats. The lateral geniculate nucleus and centrolateral nucleus were found to primarily express alpha 1G and alpha 1I, while the reticular thalamic nucleus expressed alpha 1H and alpha 1I. Expression was higher in WAG/Rij when compared to ACI. The T-type Ca2+ current properties matched the predictions derived from the expression pattern analysis. Current density was larger in all nuclei of WAG/Rij rats when compared to ACI and correlated with ITS size and the minimum LTS generating slope, while T-type Ca2+ current voltage dependency correlated with the LTS onset potential. (C) 2008 Elsevier Inc. All rights reserved.