Ginkgo biloba extract EGb 761 attenuates hippocampal neuronal loss and cognitive dysfunction resulting from chronic restraint stress in ovariectomized rats
Ginkgo biloba extract EGb 761 attenuates hippocampal neuronal loss and cognitive dysfunction resulting from chronic restraint stress in ovariectomized rats
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DOI：
10.1016/j.neuroscience.2007.07.042
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发表时间：
2007-10
期刊：
影响因子：
3.3
通讯作者：
K. Takuma;Y. Hoshina;S. Arai;Y. Himeno;A. Matsuo;Y. Funatsu;Y. Kitahara;D. Ibi;M. Hayase;H. Kamei;H. Mizoguchi;T. Nagai;K. Koike;M. Inoue;K. Yamada
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文献类型：
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作者：
K. Takuma;Y. Hoshina;S. Arai;Y. Himeno;A. Matsuo;Y. Funatsu;Y. Kitahara;D. Ibi;M. Hayase;H. Kamei;H. Mizoguchi;T. Nagai;K. Koike;M. Inoue;K. Yamada
我们最近发现，卵巢切除术（OVX）和慢性束缚应激的组合导致认知功能障碍，减少海马CA 3神经元在雌性大鼠和雌激素替代抑制OVX/应激诱导的行为和形态学变化。在这项研究中，我们研究了银杏叶提取物（EGb 761），一种流行的草药补充剂，对OVX/应激大鼠的认知功能障碍和神经形态学变化的影响。雌性Fisher 344大鼠随机分为三组：溶剂处理的OVX组、EGb 761（50 mg/kg）处理的OVX组和溶剂处理的假手术对照组。卵巢切除术后两个月，所有动物接受束缚应激21天（6小时/天），然后进行一种新的物体识别测试，然后通过尼氏染色进行形态学检查。每日一次口服EGb 761，直至进行行为分析。EGb 761治疗改善了OVX/应激组的记忆障碍和海马神经元丢失，其方式与17β-雌二醇相同。另一方面，EGb 761不影响OVX后骨密度的丢失和体重的增加，尽管17β-雌二醇减弱了它们。这些结果对EGb 761在绝经后妇女中的神经保护和认知增强作用具有重要意义，并表明这些作用是由与雌激素不同的机制介导的。
We have recently found that a combination of ovariectomy (OVX) and chronic restraint stress causes cognitive dysfunction and reduces hippocampal CA3 neurons in female rats and that estrogen replacement suppresses the OVX/stress-induced behavioral and morphological changes. In this study, we examined the effect of Ginkgo biloba extract (EGb 761), a popular herbal supplement, on the cognitive dysfunction and neuromorphological change in OVX/stress-subjected rats. Female Fisher 344 rats were randomly divided into three groups: vehicle-treated OVX, EGb 761 (50 mg/kg) -treated OVX and vehicle-treated sham-operated control groups. Two months after ovariectomy, all animals received restraint stress for 21 days (6 h/day), and were then subjected to a novel object recognition test followed by morphological examination by Nissl staining. EGb 761 was orally administered once daily until the behavioral analysis was done. Treatment with EGb 761 improved memory impairment and neuronal loss of hippocampus in the OVX/stress-subjected group in the same ways as 17β-estradiol. On the other hand, EGb 761 did not affect the loss of bone mineral density and increase in body weight after OVX, although 17β-estradiol attenuated them. These results have important implications for neuroprotective and cognition enhancing effects of EGb 761 in postmenopausal women and suggest that the effects are mediated by a different mechanism from estrogen.