Apolipoprotein E Genotypes, Lipid Peroxidation, and Antioxidant Status among Mild and Severe Preeclamptic Women from Western Iran: Protective Role of Apolipoprotein ε2 Allele in Severe Preeclampsia
Apolipoprotein E Genotypes, Lipid Peroxidation, and Antioxidant Status among Mild and Severe Preeclamptic Women from Western Iran: Protective Role of Apolipoprotein ε2 Allele in Severe Preeclampsia
复制标题
DOI：
10.3109/10641955.2012.690055
复制
发表时间：
2012-01-01
影响因子：
1.5
通讯作者：
Rahimi, Ziba
中科院分区：
文献类型：
--
作者：
Ahmadi, Reza;Rahimi, Zohreh;Rahimi, Ziba
Objective.本研究的目的是检测载脂蛋白E（APOE）基因型和氧化应激与轻度和重度先兆子痫风险的关系。方法.在一项病例对照研究中，研究了198名患有先兆子痫的妇女，包括128名轻度先兆子痫妇女和70名重度先兆子痫妇女，以及来自伊朗西部的101名对照孕妇。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法鉴定APOE基因型。分别采用高效液相色谱法和商品化试剂盒测定血清丙二醛（MDA）水平和总抗氧化能力（TAC）。结果重度子痫前期组APOE等位基因频率（2.1%）显著低于对照组（9.4%）（p = 0.008）。APOE α 2等位基因的存在与重度先兆子痫风险降低约5倍相关[ OR - 0.21（95% CI - 0.6-0.73，p - 0.014）]。重度子痫前期（10.87 ± 4.61 μ M）和轻度子痫前期（9.81 ± 3.67 μ M）孕妇血清MDA水平显著高于对照组。根据APOE等位基因，观察到血清MDA水平降低的趋势，分别为9.23、10.22和10.43 μ M，子痫前期患者血清MDA水平与HDL-C水平呈负相关（r =-0.16，p = 0.029）。血清MDA水平与舒张压呈正相关（r = 0.15，p = 0.037）。结论我们在库尔德族背景人群中的研究表明，APOE β 2等位基因对重度先兆子痫的保护作用可能是通过该等位基因的高抗氧化能力。
Objective. The aim of this study was to examine the association of apolipoprotein E (APOE) genotypes and oxidative stress with the risk of mild and severe preeclampsia. Methods. In a case-control study, 198 women with preeclampsia including 128 women with mild and 70 women with severe preeclampsia and 101 control pregnant women from Western Iran were studied. The APOE genotypes were identified using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method. The serum level of malondialdehyde (MDA) and total antioxidant capacity (TAC) were determined using high-performance liquid chromatography and commercial kits, respectively. Results. The frequency of APOE epsilon 2 allele in severe preeclamptic women (2.1%) was significantly (p = 0.008) lower than that in controls (9.4%). The presence of APOE epsilon 2 allele was associated with around five times decreased risk of severe preeclampsia [ OR - 0.21 (95% CI - 0.6-0.73, p - 0.014)]. A significantly higher serum level of MDA was observed in women with severe (10.87 +/- 4.61 mu M) and mild (9.81 +/- 3.67 mu M) preeclampsia compared with that in controls. A trend toward decrease serum level of MDA was observed according to the APOE alleles as epsilon 2 < epsilon 3 < epsilon 4 (9.23, 10.22, and 10.43 mu M, respectively). In preeclamptic women, an inverse correlation was detected between serum levels of MDA and HDL-C (r = -0.16, p = 0.029). However, there was a direct correlation between serum level of MDA with diastolic blood pressure (r = 0.15, p = 0.037). Conclusion. Our study in a population with Kurdish ethnic background indicates a protective role for APOE epsilon 2 allele against severe preeclampsia that might be through high antioxidant capacity of this allele.