Immunohistochemical localization of prostaglandin synthase in the rat uterus and embryo during the peri-implantation period.
Immunohistochemical localization of prostaglandin synthase in the rat uterus and embryo during the peri-implantation period.
复制标题
围着床期间大鼠子宫和胚胎中前列腺素合酶的免疫组织化学定位。
DOI：
10.1095/biolreprod38.2.333
复制
发表时间：
1988
影响因子：
3.6
通讯作者：
Dey,SK
中科院分区：
文献类型：
--
作者：
Parr,MB;Parr,EL;Munaretto,K;Clark,MR;Dey,SK
在许多物种中，前列腺素(PGs)似乎在子宫血管通透性增加和随后的着床蜕膜形成中起作用。然而，这些PG的生产地点尚未明确确定。为了阐明胚泡和子宫中各种细胞在着床时的PG合成能力，我们研究了妊娠第1、4、5、6和7天的大鼠胚泡和子宫中PG合成酶的免疫组织化学定位。在怀孕第一天的子宫中，PG合成酶的标记可以忽略不计。第4天，腔上皮和腺上皮、腔上皮旁的间质细胞、血管和部分白细胞中的标记增强。在第5~7天的囊胚中检测到PG合成酶，但在此期间腔上皮细胞和腺上皮细胞中的标记逐渐丢失。植入后第5天，植入部位邻近腔上皮的早期分化的基质细胞呈明亮的标记，而周围基质细胞仅有微弱的标记。到第7天，初级蜕膜区的分化细胞几乎没有标记，但次级蜕膜区的细胞被明亮地标记。这些结果表明，PG合成酶存在于大鼠胚泡和几种子宫细胞中，并且在着床过程中其在子宫细胞中的定位发生了显著的变化。
Prostaglandins (PGs) appear to have a role in the appearance of the increased uterine vascular permeability and subsequent decidualization observed at implantation in many species. However, the sites of production of these PGs have not been clearly established. To clarify the PG synthetic capacity of the blastocyst and the various types of cells in the uterus at implantation, we have studied the immunohistochemical localization of PG synthase in the rat blastocyst on Days 5 to 7 and uterus on Days 1, 4, 5, 6, and 7 of pregnancy. Labeling of PG synthase was negligible in the uterus on Day 1 of pregnancy. On Day 4, there was increased labeling in the luminal and glandular epithelium, in stromal cells adjacent to the luminal epithelium, and in blood vessels and some leukocytes. PG synthase was detected in the blastocysts on Days 5 to 7, but there was a gradual loss of label in the luminal and glandular epithelial cells during this period. Early differentiating stromal cells adjacent to the luminal epithelium in the implantation site on Day 5 showed bright labeling, whereas peripheral stromal cells were only slightly labeled. By Day 7, the differentiated cells of the primary decidual zone showed little or no label, but cells in the secondary decidual zone were brightly labeled. These results indicate that PG synthase is present in the rat blastocyst and in several kinds of uterine cells, and that its localization in uterine cells changed markedly during the implantation process.