daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans.
daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans.
复制标题
DOI：
10.1101/gad.14.12.1512
复制
发表时间：
2000-06
影响因子：
10.5
通讯作者：
Adam Antebi;Wen-Hui Yeh;D. Tait;E. Hedgecock;D. Riddle
中科院分区：
文献类型：
--
作者：
Adam Antebi;Wen-Hui Yeh;D. Tait;E. Hedgecock;D. Riddle
daf-12基因作用于调节秀丽隐杆线虫幼虫滞育、发育年龄和成虫寿命的途径的交汇处。它编码一个与两个C.线虫受体、NHR-8和NHR-48、果蝇DHR 96和脊椎动物维生素D和孕烷-X受体。daf-12具有三种预测的蛋白质同种型，其中两种含有DNA和配体结合结构域，而其中一种仅含有配体结合结构域。突变聚集在DNA和配体结合结构域中，但对应于不同的表型类别。β-12在胚胎至成体的靶组织中广泛表达，但在中期幼虫阶段上调。在成人中，表达持续存在于神经系统和体细胞性腺中，这两种组织调节成人寿命。我们建议，β-12整合激素信号的细胞目标，以协调主要的生活史性状。
The daf-12 gene acts at the convergence of pathways regulating larval diapause, developmental age, and adult longevity in Caenorhabditis elegans. It encodes a nuclear receptor most closely related to two C. elegans receptors, NHR-8 and NHR-48, Drosophila DHR96, and vertebrate vitamin D and pregnane-X receptors. daf-12 has three predicted protein isoforms, two of which contain DNA- and ligand-binding domains, and one of which contains the ligand-binding domain only. Mutations cluster in DNA- and ligand-binding domains, but correspond to distinct phenotypic classes. DAF-12 is expressed widely in target tissues from embryo to adult, but is upregulated during midlarval stages. In the adult, expression persists in nervous system and somatic gonad, two tissues that regulate adult longevity. We propose that DAF-12 integrates hormonal signals in cellular targets to coordinate major life history traits.