Helicase-contrahelicase interaction and the mechanism of termination of DNA replication.
Helicase-contrahelicase interaction and the mechanism of termination of DNA replication.
复制标题
解旋酶-反解旋酶相互作用和 DNA 复制终止机制。
DOI：
10.1016/s0092-8674(00)81995-9
复制
发表时间：
1996
期刊：
影响因子：
64.5
通讯作者：
Bastia,D
中科院分区：
文献类型：
--
作者：
Manna,AC;Pai,KS;Bussiere,DE;Davies,C;White,SW;Bastia,D
DNA复制在序列特异性复制末端的终止通过复制终止子蛋白（RTP）与末端序列的结合而增强，导致复制解旋酶的极性停滞（逆解旋酶活性）。已经提出了两种替代模型来解释复制叉停滞的机制。在第一个模型中，RTP-末端DNA相互作用简单地对解旋酶运动施加极性屏障，而不涉及解旋酶和终止子蛋白之间的任何特定相互作用。第二个模型提出，有一个特定的相互作用之间的两种蛋白质，DNA-蛋白质相互作用都限制了叉逮捕的复制末端，并决定了该过程的极性。本文提出的证据强烈支持第二种模式。
Termination of DNA replication at a sequence-specific replication terminus is potentiated by the binding of the replication terminator protein (RTP) to the terminus sequence, causing polar arrest of the replicative helicase (contrahelicase activity). Two alternative models have been proposed to explain the mechanism of replication fork arrest. In the first model, the RTP–terminus DNA interaction simply imposes a polar barrier to helicase movement without involving any specific interaction between the helicase and the terminator proteins. The second model proposes that there is a specific interaction between the two proteins, and that the DNA–protein interaction both restricts the fork arrest to the replication terminus and determines the polarity of the process. The evidence presented in this paper strongly supports the second model.