Specificity of the fatty acyl moieties of diacylglycerol for the activation of calcium-activated, phospholipid-dependent protein kinase.
Specificity of the fatty acyl moieties of diacylglycerol for the activation of calcium-activated, phospholipid-dependent protein kinase.
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二酰基甘油的脂肪酰基部分对于钙激活的磷脂依赖性蛋白激酶的激活的特异性。
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
T. Fujikura
中科院分区：
文献类型：
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作者：
T. Mori;Y. Takai;B. Yu;J. Takahashi;Y. Nishizuka;T. Fujikura
研究了二酰基甘油的脂肪酰基部分对 Ca2+ 激活的磷脂依赖性蛋白激酶的激活的特异性。先前已证明二酰基甘油可通过增加对 Ca2+ 和磷脂的亲和力来激活该酶，这两者对于酶的激活都是必不可少的。在位置1或2处含有至少一个不饱和脂肪酸的二酰基甘油在该能力中完全具有活性，而与测试范围C2至C18中的其他脂肪酰基部分的链长无关。含有两个饱和脂肪酸的二酰基甘油（例如二棕榈精和二硬脂精）的效果要差得多。单酰基甘油和三酰基甘油以及游离脂肪酸完全没有活性，表明二酰基甘油结构是必需的。
The specificity of the fatty acyl moieties of diacylglycerol for the activation of Ca2+-activated, phospholipid-dependent protein kinase was investigated. Diacylglycerol has been previously shown to activate this enzyme by increasing the affinity for Ca2+ and phospholipid, both of which are indispensable for the enzyme activation. Diacylglycerols containing at least one unsaturated fatty acid at either position 1 or 2 are fully active in this capacity, irrespective of the chain length of the other fatty acyl moiety in the range tested, C2 to C18. Diacylglycerols containing two saturated fatty acids such as dipalmitin and distearin are far less effective. Mono- and triacylglycerols and free fatty acids are totally inactive, indicating that the diacylglycerol structure is essential.