CYRI/ Fam49 Proteins Represent a New Class of Rac1 Interactors.
CYRI/ Fam49 Proteins Represent a New Class of Rac1 Interactors.
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DOI：
10.1080/19420889.2019.1643665
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发表时间：
2019-01-01
影响因子：
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通讯作者：
Machesky, Laura M
中科院分区：
文献类型：
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作者：
Whitelaw, Jamie A;Lilla, Sergio;Machesky, Laura M
Fam49 蛋白现在称为 CYRI（CYFIP 相关 Rac 相互作用蛋白），在许多门中进化上是保守的。它们的氨基酸序列最接近的是 CYFIP，因为这两种蛋白质都包含功能未知的结构域 DUF1394。我们最近表明 CYRI 和 DUF1394 可以介导与 Rac1 的结合，并且越来越多的证据表明 CYRI 在细胞迁移、趋化性和病原体进入细胞中发挥着重要作用。在这里，我们讨论 CYRI 蛋白如何通过包含 Rac1、Scar/WAVE 复合物、Arp2/3 复合物和分支肌动蛋白的正反馈环和负反馈环适应当前肌动蛋白动力学控制框架。我们还提供了有关 Rac1 和 CYRI 之间相互作用的数据，在无偏质谱筛选中寻找 Rac1 活性突变体的相互作用物。
Fam49 proteins, now referred to as CYRI (CYFIP-related Rac Interactor), are evolutionarily conserved across many phyla. Their closest relative by amino acid sequence is CYFIP, as both proteins contain a domain of unknown function DUF1394. We recently showed that CYRI and the DUF1394 can mediate binding to Rac1 and evidence is building to suggest that CYRI plays important roles in cell migration, chemotaxis and pathogen entry into cells. Here we discuss how CYRI proteins fit into the current framework of the control of actin dynamics by positive and negative feedback loops containing Rac1, the Scar/WAVE Complex, the Arp2/3 Complex and branched actin. We also provide data regarding the interaction between Rac1 and CYRI in an unbiassed mass spectrometry screen for interactors of an active mutant of Rac1.