Cholesterol synthesis in mice is suppressed but lipofuscin formation is not affected by long-term feeding of n-3 fatty acid-enriched oils compared with lard and n-6 fatty acid-enriched oils
Cholesterol synthesis in mice is suppressed but lipofuscin formation is not affected by long-term feeding of n-3 fatty acid-enriched oils compared with lard and n-6 fatty acid-enriched oils
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DOI：
10.1248/bpb.26.766
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发表时间：
2003-06-01
影响因子：
2
通讯作者：
Okuyama, H
中科院分区：
文献类型：
--
作者：
Du, CY;Sato, A;Okuyama, H
膳食多不饱和脂肪酸的低胆固醇活性在相对短期但非长期喂养后观察到，并且怀疑其长期喂养通过脂质过氧化物和年龄色素（脂褐素）的组织积累加速衰老。为了更详细地定义脂肪和油的长期影响，从17周龄到71周龄，雌性小鼠被喂食常规基础饮食，补充猪油（Lar），高亚油酸（n-6）红花油（Saf），菜籽油（Rap），高α-亚麻酸（n-3）紫苏油（Per）或二十二碳六烯酸乙酯和大豆油（DHA/大豆）的混合物。DHA/大豆和Per组与Lar和Saf组相比血清胆固醇水平降低，但Lar和Saf组之间的差异不显著。肝脏中的3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶活性在Per和DHA/大豆组中也显著较低。然而，没有显着差异的脂褐素含量在大脑和肝脏中观察到5个饮食组之间，尽管饮食和/或组织脂质的过氧化指数显着差异。这些结果表明，与动物脂肪和高亚油酸（n-6）油相比，富含n-3脂肪酸的油通过抑制肝脏HMG-CoA还原酶活性而降低胆固醇，但组织脂褐素含量不受小鼠长期喂食具有不同不饱和度的脂肪和油的影响。
Hypocholesterolemic activity of dietary polyunsaturated fatty acids is observed after relatively short-term but not long-term feedings, and their long-term feedings are suspected to accelerate aging through tissue accumulation of lipid peroxides and age pigments (lipofuscin). To define the long-term effects of fats and oils in more detail, female mice were fed a conventional basal diet supplemented with lard (Lar), high-linoleic (n-6) safflower oil (Saf), rapeseed oil (Rap), high-alpha-linolenic (n-3) perilla oil (Per), or a mixture of ethyl docosahexaenoate and soybean oil (DHA/Soy) from 17 weeks to 71 weeks of age. The DHA/Soy and Per groups had decreased serum cholesterol levels compared with the Lar and Saf groups, but the difference between the Lar and Saf groups was not significant. The 3-hydroxy-3-methyglutary-CoA (HMG-CoA) reductase activity in the liver was also significantly lower in the Per and DHA/Soy groups. However, no significant difference in lipofuscin contents in the brain and liver was observed among the 5 dietary groups, despite significant differences in peroxidizability indices of the dietary and/or tissue lipids. These results indicate that n-3 fatty acid-rich oils are hypocholesterolemic by suppressing hepatic HMG-CoA reductase activity compared with animal fats and high-linoleic (n-6) oil, but tissue lipofuscin contents are not affected by a long-term feeding of fats and oils with different degree of unsaturation in mice.