Distribution of PDIA3 transcript and protein in rat testis and sperm cells.
Distribution of PDIA3 transcript and protein in rat testis and sperm cells.
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PDIA3转录物和蛋白质在大鼠睾丸和精子细胞中的分布。
DOI：
10.1111/j.1439-0531.2012.02024.x
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
W. Xing
中科院分区：
文献类型：
--
作者：
X. Zhao;R. Tang;M. Wang;W. Guo;J. Liu;L. Li;W. Xing
蛋白质二硫键异构酶家族 A3 (PDIA3) 具有硫氧还蛋白活性，广泛表达于多种组织中并参与多种细胞过程。最近在人类和小鼠的精子细胞中发现了它，并且可以影响精卵融合。因此，本研究旨在鉴定大鼠睾丸和精子细胞中的PDIA3 mRNA和蛋白质。通过RT-PCR克隆大鼠PDIA3 cDNA。 cRNA 核糖探针从 PDIA3 cDNA 转录而来，并用于通过原位杂交显示大鼠睾丸中 PDIA3 mRNA 的位置。通过免疫组织化学和免疫细胞化学还分别观察了睾丸和精子细胞中的 PDIA3 蛋白分布。大鼠PDIA3转录本和蛋白定位于大鼠睾丸精母细胞至精子期的细胞中，大部分位于粗线期精母细胞中。在完整的精子膜（包括尾部）上也观察到了 PDIA3 蛋白。大鼠PDIA3基因的转录和翻译贯穿整个精子发生过程，PDIA3蛋白遍布精子细胞膜。该结果为进一步研究PDIA3在大鼠精子发生和精卵融合中的功能提供了睾丸和精子中PDIA3的一些初步信息。
Protein disulphide isomerase family A3 (PDIA3) has an activity of thioredoxin, widely expressed in multiple tissues and involved in multiple cellular processes. It was recently found in human and mouse sperm cells and could affect sperm-egg fusion. Therefore, the present investigation aims to identify PDIA3 mRNA and protein in rat testis and sperm cells. Rat PDIA3 cDNA was cloned by RT-PCR. The cRNA riboprobe was transcribed from PDIA3 cDNA and was used to display PDIA3 mRNA location in rat testes by in situ hybridization. PDIA3 protein distribution was also observed in testis and sperm cells by immunohistochemistry and immunocytochemistry, respectively. The rat PDIA3 transcript and protein were localized in the cells from spermatocytes to the spermatozoa phases of rat testes, mostly in the pachytene spermatocytes. PDIA3 protein was also observed on the intact sperm membrane including the tail. The rat PDIA3 gene is transcribed and translated through the whole spermatogenesis process, and the PDIA3 protein is spread all over the sperm cell membrane. The results provide some primary information about PDIA3 in testis and sperm for further study on PDIA3 function in rat spermatogenesis and sperm-egg fusion.
影响因子：
6.6
作者：
Kim-Han, Jeong Sook;O'Malley, Karen L.
通讯作者：
O'Malley, Karen L.
影响因子：
2.7
作者：
Da Ros, Vanina G.;Maldera, Julieta A.;Cuasnicu, Patricia S.
通讯作者：
Cuasnicu, Patricia S.
影响因子：
11.8
作者：
Ellerman, Diego A.;Myles, Diana G.;Primakoff, Paul
通讯作者：
Primakoff, Paul