CRISPR/Cas9-Mediated Gene Editing of BnFAD2 and BnFAE1 Modifies Fatty Acid Profiles in Brassica napus
CRISPR/Cas9-Mediated Gene Editing of BnFAD2 and BnFAE1 Modifies Fatty Acid Profiles in Brassica napus
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DOI：
10.3390/genes13101681
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
张尧峰
中科院分区：
文献类型：
--
作者：
石江华;倪西源;黄吉祥;傅鹰;王枟刘;余华胜;张尧峰
Fatty acid (FA) composition determines the quality of oil from oilseed crops, and thus.is a major target for genetic improvement. FAD2 (Fatty acid dehydrogenase 2) and FAE1 (fatty acid.elongase 1) are critical FA synthetic genes, and have been the focus of genetic manipulation to alter.fatty acid composition in oilseed plants. In this study, to improve the nutritional quality of rapeseed.cultivar CY2 (about 50% oil content; of which 40% erucic acid), we generated novel knockout plants.by CRISPR/Cas9 mediated genome editing of BnFAD2 and BnFAE1 genes. Two guide RNAs were.designed to target one copy of the BnFAD2 gene and two copies of the BnFAE1 gene, respectively. A.number of lines with mutations at three target sites of BnFAD2 and BnFAE1 genes were identified.by sequence analysis. Three of these lines showed mutations in all three target sites of the BnFAD2.and BnFAE1 genes. Fatty acid composition analysis of seeds revealed that mutations at all three.sites resulted in significantly increased oleic acid (70–80%) content compared with that of CY2 (20%),.greatly reduced erucic acid levels and slightly decreased polyunsaturated fatty acids content. Our.results confirmed that the CRISPR/Cas9 system is an effective tool for improving this important trait.
