Nitrogen removal from wastewater by anaerobic methane-driven denitrification in a lab-scale reactor: heterotrophic denitrifiers associated with denitrifying methanotrophs
Nitrogen removal from wastewater by anaerobic methane-driven denitrification in a lab-scale reactor: heterotrophic denitrifiers associated with denitrifying methanotrophs
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在实验室规模反应器中通过厌氧甲烷驱动的反硝化从废水中脱氮：与反硝化甲烷氧化菌相关的异养反硝化菌
DOI：
10.1007/s00253-015-6939-9
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发表时间：
2015-12-01
影响因子：
5
通讯作者：
Hu, Baolan
中科院分区：
文献类型：
--
作者：
He, Zhanfei;Wang, Jiaqi;Hu, Baolan
亚硝酸盐依赖的厌氧甲烷氧化是一种新发现的以甲烷为电子供体将亚硝酸盐还原为二氮的生物过程，具有良好的脱氮潜力。在这项工作中，一个实验室规模的序批式反应器（SBR）运行了609天，甲烷作为唯一的外部电子供体。在SBR中，合成废水中的亚硝酸盐被连续去除;最终体积氮去除率为12.22±0.02 mg N L-1day-1，氮去除百分比为98.5 ± 0.2%。微生物群落分析表明，最终污泥中的甲烷氧化菌占主导地位（60- 70%）。活性测试和微生物分析均表明，在整个运行过程中，反应器中均存在异养微生物。609 d后的活性测试表明，异养反硝化的脱氮率为17 ± 2%，n-damo的脱氮率为83 ± 2%.优势甲烷氧化菌和相关异养菌之间可能通过交叉摄食形成一种互利共生关系。异养生物可以依靠分解甲烷氧化菌分泌的微生物产物生存，并提供分解甲烷氧化菌所需的生长因子。
Nitrite-dependent anaerobic methane oxidation (n-damo) is a newly discovered bioprocess that reduces nitrite to dinitrogen with methane as electron donor, which has promising potential to remove nitrogen from wastewater. In this work, a lab-scale sequencing batch reactor (SBR) was operated for 609 days with methane as the sole external electron donor. In the SBR, nitrite in synthetic wastewater was removed continuously; the final volumetric nitrogen removal rate was 12.22±0.02 mg N L−1day−1and the percentage of nitrogen removal was 98.5 ± 0.2 %. Microbial community analysis indicated that denitrifying methanotrophs dominated (60–70 %) the population of the final sludge. Notably, activity testing and microbial analysis both suggested that heterotrophic denitrifiers existed in the reactor throughout the operation period. After 609 days, the activity testing indicated the nitrogen removal percentage of heterotrophic denitrification was 17 ± 2 % and that of n-damo was 83 ± 2 %. A possible mutualism may be developed between the dominated denitrifying methanotrophs and the associated heterotrophs through cross-feed. Heterotrophs may live on the microbial products excreted by denitrifying methanotrophs and provide growth factors that are required by denitrifying methanotrophs.