The Expression of Glypican-3 in Colorectal Cancer
The Expression of Glypican-3 in Colorectal Cancer
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DOI：
10.3103/s0095452719050037
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发表时间：
2019-09-01
影响因子：
0.5
通讯作者：
Amini, Razieh
中科院分区：
文献类型：
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作者：
Azizpour, Sonia;Ezati, Razieh;Amini, Razieh
磷脂酰肌醇蛋白聚糖-3（GPC 3），硫酸乙酰肝素蛋白聚糖是一种新兴的肿瘤标志物，它的过度表达已在几种类型的癌症，如肝细胞癌（HCC），黑色素瘤等。在这项研究中，GPC 3 mRNA的表达在61人结直肠癌（CRC）组织和61例正常的癌旁组织，使用实时荧光PCR检测。使用免疫组织化学（IHC），在显示与正常组织相比肿瘤组织中GPC 3 mRNA表达显著升高的病例中检查GPC 3蛋白的表达。与正常癌旁组织相比，49.2%的CRC肿瘤病例中GPC 3基因表达显著增加。GPC 3 mRNA的表达与患者的病理分化程度及年龄有关。有趣的是，与正常邻近组织相比，在接受新辅助化疗的16名患者的肿瘤组织中发现GPC 3 mRNA的表达显著降低。出乎意料的是，在30个组织中通过IHC未检测到GPC 3蛋白，显示GPC 3 mRNA表达上调。我们的结果表明，GPC 3在mRNA水平上可以在大肠癌组织中表达，而在蛋白水平上未发现其表达。
Glypican-3 (GPC3), a heparan sulfate proteoglycan is an emerging tumor marker; its overexpression has been stated in several types of cancer such as hepatocellular carcinoma (HCC), melanoma and etc. In this study, the expression of the GPC3 mRNA was investigated in 61 human colorectal cancer (CRC) tissues and 61 normal adjacent tissues, using the real-time PCR assay. Using immunohistochemistry (IHC), the expression of the GPC3 protein was examined in the cases that showed a marked elevation in the expression of the GPC3 mRNA in the tumor tissues, compared to the normal tissues. A significant increase in the expression of the GPC3 gene was revealed in 49.2% of the cases of CRC tumors, compared to the normal adjacent tissues. The expression of the GPC3 mRNA was found to significantly correlate with the pathological differentiation and the age of the patients. Interestingly, a significant reduction in the expression of the GPC3 mRNA was found in the tumor tissues of the 16 patients, who underwent the neoadjuvant chemotherapy, compared to the normal adjacent tissues. Unexpectedly, the GPC3 protein was not detected by IHC in 30 tissues, exhibiting the upregulation in the expression of the GPC3 mRNA. Our results indicated that GPC3 could be expressed in the CRC tissues at the mRNA level, while its expression could not be found at the protein level.