Association between cathepsin L (CTSL) and cathepsin S (CTSS) polymorphisms and meat production and carcass traits in Italian Large White pigs
Association between cathepsin L (CTSL) and cathepsin S (CTSS) polymorphisms and meat production and carcass traits in Italian Large White pigs
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DOI：
10.1016/j.meatsci.2010.01.023
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发表时间：
2010-06-01
期刊：
影响因子：
7.1
通讯作者：
Russo, V.
中科院分区：
文献类型：
--
作者：
Fontanesi, L.;Speroni, C.;Russo, V.
本研究旨在鉴定和分析意大利大白猪组织蛋白酶L(Ctsl)和组织蛋白酶S(Ctss)两个组织蛋白酶基因的DNA标记，并研究它们与肉质参数(包括宰后肌肉组织蛋白酶活性)和几个生产性状的相关性。在对猪CTSL和CTSS基因的电子分析中，发现了两个新的单核苷酸多态(SNPs)，一个位于外显子5(CTSL)，另一个位于5‘-非翻译区(CTSS)。对这两个SNPs在7个不同猪品种中的等位基因频率进行了研究。此外，辐射杂交定位将CTSS基因定位在猪的4号染色体上，这是一个重要的肉和脂肪沉积QTL区域。对268头意大利大白猪的肉质性状进行了关联分析，结果表明，CTSL基因多态与平均日增重(ADG)、瘦肉量(LC)和背脂厚度(BFT)的育种值相关(P<；0.05)。在其他意大利大白猪中，LC和BFT的结果也得到了选择性基因分型方法的证实(P<；0.01)。在268头意大利大白猪中，CTSS基因多态性与料重比和日增重相关(P<；0.05)。所分析的标记与肉质参数(pH(1)、pH(U)、乳酸、糖原、糖酵解潜力和组织蛋白酶活性，都是在半膜肌上测量的)之间没有关联。总体而言，这些结果，加上之前报道的该家族其他基因的结果，表明组织蛋白酶基因在定义猪的重要经济性状方面发挥了作用。(C)2010爱思唯尔有限公司。保留所有权利。
The aim of this work was to identify and analyse DNA markers in two cathepsin genes, cathepsin L (CTSL) and cathepsin S (CTSS), and study their association with meat quality parameters (including cathepsin activity of post mortem muscle) and several production traits in Italian Large White pigs. In silico analysis of the porcine CTSL and CTSS genes identified two new single nucleotide polymorphisms (SNPs), one located in exon 5 (CTSL) and one positioned in the 5'-untranslated region (CTSS). Allele frequencies of these two SNPs were evaluated in 7 different pig breeds. In addition, radiation hybrid mapping localized the CTSS gene on porcine chromosome 4, in an important QTL region for meat and fat deposition. Association analysis with meat quality traits, carried out in 268 Italian Large White pigs, indicated association of the CTSL polymorphism with average daily gain (ADG), weight of lean cuts (LC) and back fat thickness (BFT) estimated breeding values (P < 0.05). Results for LC and BFT were also confirmed using a selective genotyping approach in other Italian Large White pigs (P < 0.01). In the 268 Italian Large White pigs, the CTSS polymorphism was associated with feed:gain ratio and ADG (P < 0.05). No association was observed between the analysed markers and meat quality parameters (pH(1), pH(u), lactate, glycogen, glycolytic potential and cathepsin activity, all measured on Musculus semimembranosus). Overall these results, together with those previously reported for other genes of this family, suggest that cathepsin genes play a role in defining economically important traits in pigs. (C) 2010 Elsevier Ltd. All rights reserved.