Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars
Stepwise enzymatic hydrolysis of alkaline oxidation treated sugarcane bagasse for the co-production of functional xylo-oligosaccharides and fermentable sugars
复制标题
逐步酶解碱性氧化处理的甘蔗渣，联产功能性低聚木糖和可发酵糖
DOI：
10.1016/j.biortech.2018.12.063
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发表时间：
2019-03-01
影响因子：
11.4
通讯作者：
Chen, Xinde
中科院分区：
文献类型：
--
作者：
Li, Hailong;Chen, Xindong;Chen, Xinde
利用木质纤维素生物质中提取的木聚糖酶法生产低聚木糖（XOS）需要高的化学输入。本研究以碱氧化处理后的甘蔗渣为原料，采用酶法直接水解制备低聚木糖。研究了AO预处理对SCB化学组成和水解性能的影响。采用正交设计法优化了低投料量的AO预处理工艺条件。采用木聚糖酶和纤维素酶对AO预处理的SCB进行分步酶解，得到1.78g/L的XOS，纤维素转化率由84.97%提高到91.51%。HPLC-UV和MALDI-TOF-MS分析表明，所得XOS产物主要由木二糖和木糖组成，并含有少量阿拉伯糖/4-O-甲基葡萄糖醛酸取代的木三糖和木四糖。提出的SCB联产功能性低聚木糖和可发酵糖的策略具有工业应用潜力。
High chemical input is required for enzymatic production of xylo-oligosaccharides (XOS) using xylan extracted from lignocellulosic biomass. In this study, enzymatic hydrolysis of alkaline oxidation (AO) treated sugarcane bagasse (SCB) directly for the production of XOS was conducted. The effect of AO pretreatment on the chemical compositions and hydrolytic properties of SCB was investigated. The AO pretreatment conditions with low chemical input for the production of XOS were optimized by orthogonal design. Stepwise enzymatic hydrolysis of AO pretreated SCB with xylanase and cellulase produced XOS (1.78 g/L), meanwhile, the cellulose conversion increased from 84.97% to 91.51% compared with directly enzymatic hydrolysis using cellulase. HPLC-UV and MALDI-TOF-MS analysis indicated that the obtained XOS products were mainly composed of xylobiose and xylose with a small amount of arabinose/4-O-methylglucuronic acid substituted xylotriose and xylotetraose. The proposed strategy for the co-production of functional XOS and fermentable sugars from SCB showed potential of industrial application.