Overview of the retrovirus transduction system.
Overview of the retrovirus transduction system.
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DOI：
10.1002/0471142727.mb0909s36
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Pear, W
中科院分区：
文献类型：
--
作者：
Cepko, C;Pear, W
逆转录病毒载体是用于在体内或体外将非病毒基因引入有丝分裂细胞的感染性病毒。利用天然存在的逆转录病毒的有效和精确的整合机制来产生稳定整合到宿主染色体中的病毒基因组的单个拷贝或几个拷贝。本单元概述了逆转录病毒的生命周期和载体设计和包装细胞系的描述。
A retrovirus vector is an infectious virus used to introduce a nonviral gene into mitotic cells in vivo or in vitro. The efficient and precise integration machinery of naturally occurring retroviruses is utilized to produce either a single copy or a few copies of the viral genome stably integrated into a host chromosome. This unit presents an overview of the retrovirus life cycle and a description of vector designs and packaging cell lines.