In Vivo Imaging of Venous Thrombus and Pulmonary Embolism Using Novel Murine Venous Thromboembolism Model
In Vivo Imaging of Venous Thrombus and Pulmonary Embolism Using Novel Murine Venous Thromboembolism Model
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DOI：
10.1016/j.jacbts.2020.01.010
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发表时间：
2020-04-01
影响因子：
9.7
通讯作者：
Hirata, Ken-ichi
中科院分区：
文献类型：
--
作者：
Okano, Mitsumasa;Hara, Tetsuya;Hirata, Ken-ichi
本工作建立了一种新的小鼠静脉血栓栓塞（VTE）模型。该模型具有代表临床静脉血栓形成的多个新特征，包括：1)深静脉血栓形成并在股静脉/隐静脉长轴延伸；2)静脉瓣袋内形成血栓；3)富纤维蛋白深静脉血栓缝合线诱导自发性肺栓塞切除；4)无心脏运动的股静脉/隐静脉可以实现富含纤维蛋白的深静脉血栓的高分辨率活体显微成像。这种新模型只需要市售的荧光显微镜。因此，该模型对于更好地理解静脉血栓栓塞的病理生理具有重要的潜力。(C) 2020作者。由爱思唯尔代表美国心脏病学会基金会出版。
This work established a new murine venous thromboembolism (VTE) model. This model has multiple novel features representing clinical VTE that include the following: 1) deep venous thrombosis (DVT) was formed and extended in the long axis of femoral/saphenous vein; 2) thrombus was formed in a venous valve pocket; 3) deligation of suture-induced spontaneous pulmonary emboli of fibrin-rich DVT; and 4) cardiac motion-free femoral/saphenous vein allowed high-resolution intravital microscopic imaging of fibrin-rich DVT. This new model requires only commercially available epifluorescence microscopy. Therefore, this model has significant potential for better understanding of VTE pathophysiology. (C) 2020 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation.