Baseline susceptibility and changes in susceptibility to Bacillus thuringiensis subsp. kurstaki under selection pressure in European corn borer (Lepidoptera: Pyralidae)
Baseline susceptibility and changes in susceptibility to Bacillus thuringiensis subsp. kurstaki under selection pressure in European corn borer (Lepidoptera: Pyralidae)
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DOI：
10.1093/jee/90.5.1137
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发表时间：
1997-10-01
影响因子：
2.2
通讯作者：
Buschman, LL
中科院分区：
文献类型：
--
作者：
Huang, FN;Higgins, RA;Buschman, LL
欧洲玉米螟幼虫对苏云金芽孢杆菌柏林亚种的商业制剂的基线敏感性。kurstaki（Dipel ES），对来自3个不同地理位置的菌落进行测定。在堪萨斯东北部的曼哈顿附近收集的KS-NE菌落比在堪萨斯中南部的圣约翰附近收集的菌落（KS-SC菌落）和由美国农业部玉米昆虫实验室在爱荷华州艾姆斯提供的菌落（爱荷华州菌落）对Dipel更耐受。五个殖民地的欧洲玉米螟暴露于实验室选择使用mercanti饮食纳入Dipel ES。结果表明，在每个选择的群体中，欧洲玉米螟对Dipel ES的敏感性降低。在堪萨斯中南部收集的殖民地（KS-SC）中，特定浓度下幼虫的LC 50和存活率比其他殖民地增加得更快。经3代选择后，该菌落的LC_（50）增加了35.8倍，经7代选择后，LC_（50）增加了72.9倍。经过9-14代的选择，爱荷华州的3个菌落的LC（50）增加了25.0 ~ 35.2倍。在堪萨斯东北部采集的殖民地，经过4代选择，LC（50）增加了16.2倍，经过6代选择，LC（50）增加了35倍。这表明，欧洲玉米螟种群可以迅速响应强烈的选择压力与商业配方的苏云金芽孢杆菌。5个菌落的LC（90）s也显著增加，其模式与LC（50）s中观察到的模式相似，但LC（90）s的增加幅度不如LC（50）s的增加幅度大。生物测定数据表明，每公斤饲料约2.4 ml Dipel的剂量可用作初步区分剂量。从现场收集的昆虫的新生儿可以放在饮食中，将这种浓度的Dipel监测欧洲玉米螟田间种群之间的易感性的变化和区域差异。遗传基础的研究，作用机制，随着时间的推移（在没有选择压力）的这种变化的敏感性仍有待进行。
Baseline susceptibility of European corn borer larvae, Ostrinia nubilalis (Hubner), to a commercial formulation of Bacillus thuringiensis Berliner Subsp. kurstaki (Dipel ES), was determined for colonies from 3 different geographic locations. The KS-NE colony, collected near Manhattan in northeast Kansas, was more tolerant to Dipel than those collected from near St. John in south central Kansas (KS-SC colony) and colonies provided by the USDA Corn Insects Laboratory in Ames, IA (Iowa colony). Five colonies of European corn borers were exposed to laboratory selection using a meridic diet incorporating Dipel ES. Decreasing susceptibility of European corn borer to Dipel ES was observed in each colony selected. In the colony collected in south central Kansas (KS-SC), the LC50 and survival of larvae at specific concentrations increased more rapidly than it did in the other colonies. After only 3 selected generations, the LC50 of this colony increased 35.8-fold, and after 7 selected generations, it reached 72.9-fold. The LC(50)s of the 3 Iowa colonies increased 25.0- to 35.2-fold after 9-14 selected generations. The LC(50)s of the colony collected in northeast Kansas increased 16.2-fold after 4 selected generations and it reached 35-fold after 6 selected generations. This indicates that European corn borer populations can respond rapidly to intense selection pressure with a commercial formulation of Bacillus thuringiensis. The LC(90)s of the 5 colonies also increased significantly in a pattern similar to that observed in the LC(50)s, but the increases in LC(90)s were not as large as the increases for the LC(50)s. The bioassay data suggest that a dose of approximate to 2.4 mi Dipel per kilogram of diet could be used as a preliminary discriminating dose. Neonates from field-collected insects could be placed on diets incorporating this concentration of Dipel to monitor for changes and regional differences in susceptibility among field populations of European corn borer. Studies on the genetic basis, the mechanism of action, and the stability over time (in the absence of selection pressure) of this change in susceptibility remain to be carried out.