TGFβ1 contributes to cardiomyogenic-like differentiation of human bone marrow mesenchymal stem cells
TGFβ1 contributes to cardiomyogenic-like differentiation of human bone marrow mesenchymal stem cells
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DOI：
10.1016/j.ijcard.2011.08.003
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发表时间：
2013-02-10
影响因子：
3.5
通讯作者：
Airan, Balram
中科院分区：
文献类型：
--
作者：
Mohanty, Sujata;Bose, Sushmita;Airan, Balram
背景：大多数MSC心肌细胞分化方案使用5-氮杂胞苷作为诱导剂。由于转化生长因子β 1和5-氮胞苷具有相似的靶信号通路，我们研究了转化生长因子β 1是否在骨髓源的人间充质干细胞的心脏分化过程中发挥作用。方法：比较转化生长因子β 1与5-氮杂胞苷在这些细胞中的分化方案。用逆转录酶PCR、流式细胞术和定量PCR对两种分化机制进行比较。结果：我们观察到两种情况下，获得的形态特征相似。蛋白质和基因表达分析也表明在两种分化条件下心脏标志物表达谱相似。此外，转化生长因子β 1和5-氮杂胞苷可以获得相当水平的心脏细胞样分子特征，这一点通过对肌球蛋白轻链2v表达的评估得到了证实。结论：由此可见，转化生长因子β 1在人骨髓间充质干细胞的心脏分化过程中发挥了类似的作用。2011爱思唯尔爱尔兰有限公司版权所有。
Background: The majority of the protocols for cardiomyocyte differentiation of MSC use 5-azacytidine as an inducer. As transforming growth factor beta 1 and 5-azacytidine share similar target signaling pathways, we examined whether transforming growth factor beta 1 can play a role in cardiac differentiation process in human mesenchymal stem cell of bone marrow origin.Methods: The differentiation protocol involving transforming growth factor beta 1 was compared with that of 5-azacytidine in these cells. The two differentiation regimes were compared using reverse transcriptase PCR, flow cytometry, and quantitative PCR.Results: We observed that in both cases, acquired morphological features were similar. Protein and gene expression assays also indicated similar cardiac marker expression profile in both the differentiation conditions. Furthermore, transforming growth factor beta 1 and 5-azacytidine allowed the acquisition of comparable levels of cardiac cell like molecular characteristic as attested by evaluation of myosin light chain-2v expression.Conclusion: In conclusion, we demonstrate that transforming growth factor beta 1 can play a similar role in cardiac differentiation process of human bone marrow mesenchymal stem cells. (C) 2011 Elsevier Ireland Ltd. All rights reserved.