Effect of adsorbed von Willebrand factor and fibrinogen on platelet interactions with synthetic materials under flow conditions.
Effect of adsorbed von Willebrand factor and fibrinogen on platelet interactions with synthetic materials under flow conditions.
复制标题
流动条件下吸附的冯维勒布兰德因子和纤维蛋白原对血小板与合成材料相互作用的影响。
DOI：
10.1002/jbm.a.31505
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Horbett,ThomasA
中科院分区：
文献类型：
--
作者：
Wu,Yuguang;Zhang,Min;Hauch,KipD;Horbett,ThomasA
研究了吸附纤维蛋白原(FG)和血管性假血友病因子(VWF)对几种材料在低和高剪切速率下对血小板粘附性的影响。所研究的材料是聚对苯二甲酸乙二酯(PET)、聚苯乙烯(PS)、玻璃和四甘醇包覆的PET。将材料与正常血浆、血清、补充不同量Fg的Fg缺乏血浆以及添加或不添加VWF的VWF缺陷血浆进行预吸附。用Fg缺乏的血浆或血清预吸附的PET在低或高剪切率下对血小板的粘附性都很低，但随着预吸附介质中Fg的加入而增加。然而，在较高的剪切速率下，添加FG对粘附性的影响在与血浆预吸附的表面上比对血清的影响要大得多，这可能是由于血清中的VWF浓度比血浆低得多。正常等离子体预吸附的聚苯乙烯或玻璃表面在高剪切条件下的粘附性远高于低剪切条件下的粘附性，但用VWF缺乏的等离子体预吸附这些表面时，高剪切速率下的粘附性明显低于低剪切条件下的粘附性。加入VWF的缺乏VWF的血浆预吸附在聚苯乙烯上的血小板在高剪切速率下的粘附率高于低剪切速率。这些结果表明，在高剪切速率下，FG和VWF都是合成生物材料上的血小板黏附所必需的。结果表明，开发吸附少量VWF的表面是改善生物材料血液相容性的一种好方法。《生物医学杂志》85A：829-839,2008
The effect of adsorbed fibrinogen (Fg) and von Willebrand factor (vWf) on platelet adhesion at low or high shear rate to several materials was studied. The materials studied were polyethylene terephthalate (PET), polystyrene (PS), glass, and tetraglyme-coated PET. The materials were preadsorbed with normal plasma, serum, and Fgdeficient plasma replenished with various amounts of Fg, and vWf-deficient plasma with or without added vWf. Platelet adhesion to PET preadsorbed with Fg-deficient plasma or serum was low at either low or high shear rate, but increased as Fg was added to the preadsorption media. However, the effect of added Fg on adhesion at the higher shear rate was much greater on surfaces preadsorbed with plasma than for serum, probably due to the much lower vWf concentration in serum in comparison to plasma. Platelet adhesion to either polystyrene or glass preadsorbed with normal plasma was much higher at high shear than low shear, but when vWf-deficient plasma was used to preadsorb these surfaces, adhesion was much less at the higher shear rate than at low shear rate. Platelet adhesion to polystyrene preadsorbed with vWf-deficient plasma to which vWf was added was higher at high shear rate than low shear rate. These results show that under high shear rate, both Fg and vWf are required for platelet adhesion on synthetic biomaterials. The results suggest that developing surfaces that adsorb low amounts of vWf is a good approach to improving the blood compatibility of biomaterials. Ó 2007 Wiley Periodicals, Inc. J Biomed Mater Res 85A: 829–839, 2008