See-Through Technology for Biological Tissue: 3-Dimensional Visualization of Macromolecules.
See-Through Technology for Biological Tissue: 3-Dimensional Visualization of Macromolecules.
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DOI：
10.5213/inj.1632630.315
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发表时间：
2016-05
影响因子：
2.3
通讯作者：
Sun W
中科院分区：
文献类型：
--
作者：
Lee E;Kim HJ;Sun W
组织清除技术是目前生物医学科学中发展最快的领域之一。组织清除技术已成为进一步了解完整生物组织结构信息的有力手段。此外，组织清除和光学方面的技术进步使神经网络能够以亚细胞分辨率在整个脑组织中可视化。本文对各种组织清除技术进行了综述，重点介绍了组织水凝胶介导的组织清除方法，并讨论了透明组织在临床诊断中的主要优势和局限性。
Tissue clearing technology is currently one of the fastest growing fields in biomedical sciences. Tissue clearing techniques have become a powerful approach to understand further the structural information of intact biological tissues. Moreover, technological improvements in tissue clearing and optics allowed the visualization of neural network in the whole brain tissue with subcellular resolution. Here, we described an overview of various tissue-clearing techniques, with focus on the tissue-hydrogel mediated clearing methods, and discussed the main advantages and limitations of transparent tissue for clinical diagnosis.