Development of quantitative assay for tissue levels of dolichyl phosphate.
Development of quantitative assay for tissue levels of dolichyl phosphate.
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磷酸多乙醇酯组织水平定量测定的开发。
DOI：
10.1021/bi00523a028
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
AdairJr,WL
中科院分区：
文献类型：
--
作者：
Keller,RK;Tamkun,JW;AdairJr,WL
R. Kennedy Keller,* John W. Tamkun, 1* and W. Lee Adair, Jr. abstract: A sensitive assay is described for quantitating dolichyl phosphate (Dol-P), the polyprenyl phospholipid which participates in N-linked glycosylation. The assay is based on a novel reaction of alkyl phosphates with phenyl chloroformate in which a monosubstituted mixed anhydride of phosphoric and carbonic acid is formed. Evidence in support of the proposed structure for the derivative includes a phosphate to phenyl ratio of 1, infrared spectra, elemental analysis, behavior during ion-exchange chromatography, and reactivity with primary and secondary amines. A simple, rapid procedure is described for the preparation of [14C] phenyl chloroformate from [14C] phenol; use of the radiolabeled reagent allows assay of Dol-Pin the subnanomolar range. The assay was appliedIV^ Iany tissues (eg, liver, mammary gland, and oviduct) exhibit the capacity for rapid induction of N-linked glycoprotein synthesis, and thus a mechanism must exist which allows the cell to deal with an increased demand for dolichol-mediated oligosaccharide transfer. Several studies in recent years have suggested that the cell responds by aug-menting the level of available dolichyl phosphate. The work of Lucas & Levin (1977) and Lucas (1979) with hen oviduct
