RNA Recognition-like Motifs Activate a Mitogen-Activated Protein Kinase.
RNA Recognition-like Motifs Activate a Mitogen-Activated Protein Kinase.
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RNA 识别样基序激活丝裂原激活蛋白激酶。
DOI：
10.1021/acs.biochem.8b01032
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发表时间：
2018
期刊：
影响因子：
2.9
通讯作者：
Winter,Edward
中科院分区：
文献类型：
--
作者：
Phillips,Timothy;Tio,ChongWai;Omerza,Gregory;Rimal,Abhimannyu;Lokareddy,RaviK;Cingolani,Gino;Winter,Edward
Smk1 是酿酒酵母中的丝裂原激活蛋白激酶 (MAPK) 家族成员，控制孢子形成的减数分裂后程序。 Ssp2 是一种减数分裂特异性蛋白，可激活 Smk1 并触发其激活环的自磷酸化。足以激活 Smk1 的 Ssp2 片段包含两个类似于 RNA 识别基序 (RRM) 的片段。这些基序中的任何一个的突变都会消除 Ssp2 激活 Smk1 的能力。相比之下，连接 RRM 样基序的片段内的删除和插入仅部分降低了 Ssp2 的活性。此外，当这两个类似 RRM 的基序在细菌中表达为单独的蛋白质时，它们会激活 Smk1。我们还发现这两个基序都可以与 Smk1 交联，并且至少其中一个基序结合在 MAPK 的 ATP 结合袋附近。这些发现表明与 RRM 相关的基序可以直接激活蛋白激酶。
Smk1 is a mitogen-activated protein kinase (MAPK) family member in the yeastSaccharomyces cerevisiaethat controls the postmeiotic program of spore formation. Ssp2 is a meiosis-specific protein that activates Smk1 and triggers the autophosphorylation of its activation loop. A fragment of Ssp2 that is sufficient to activate Smk1 contains two segments that resemble RNA recognition motifs (RRMs). Mutations in either of these motifs eliminated Ssp2’s ability to activate Smk1. In contrast, deletions and insertions within the segment linking the RRM-like motifs only partially reduced the activity of Ssp2. Moreover, when the two RRM-like motifs were expressed as separate proteins in bacteria, they activated Smk1. We also find that both motifs can be cross-linked to Smk1 and that at least one of the motifs binds near the ATP-binding pocket of the MAPK. These findings demonstrate that motifs related to RRMs can directly activate protein kinases.