BEHAVIORAL RECOVERY FOLLOWING TRANSPLANTATION OF SUBSTANTIA NIGRA IN RATS SUBJECTED TO 6-OHDA LESIONS OF THE NIGROSTRIATAL PATHWAY .1. UNILATERAL LESIONS
BEHAVIORAL RECOVERY FOLLOWING TRANSPLANTATION OF SUBSTANTIA NIGRA IN RATS SUBJECTED TO 6-OHDA LESIONS OF THE NIGROSTRIATAL PATHWAY .1. UNILATERAL LESIONS
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DOI：
10.1016/0006-8993(81)90498-4
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
IVERSEN, SD
中科院分区：
文献类型：
--
作者：
DUNNETT, SB;BJORKLUND, A;IVERSEN, SD
在成年大鼠中研究了胚胎黑质移植物补偿由黑质纹状体多巴胺通路的单侧破坏引起的行为缺陷的能力。6天后单侧6-OHDA [6-羟基多巴胺]病变的黑质纹状体通路，病变的充分性进行了评估，通过测量同侧安非他明诱导的旋转强度。所有大鼠在损伤侧的尾壳核头部上方的皮质中接受手术腔。在51只大鼠中，将胚胎黑质移植物放置在尾壳核的背侧表面上，其余19只大鼠作为单侧损伤对照。行为测试进行了约3个月。移植后：单独的移植动物显示出由5 mg/kg安非他明诱导的同侧旋转的显著减少（补偿）;虽然移植和对照大鼠在0.25 mg/kg阿扑吗啡剂量下表现出相等的对侧旋转，但仅移植动物在0.05 mg/kg的较低剂量下表现出旋转显著减少;移植组大鼠自发旋转行为的不对称性较对照组明显降低，轻度夹尾可进一步降低不对称性;当在T-迷宫中测试自发选择行为时，对照大鼠显示出对6-OHDA损伤同侧臂的97%选择;移植大鼠在苯丙胺旋转试验中有30-40%的选择转向对侧;在感觉运动测试组合中，无论是自发测试还是在轻度夹尾诱导的激活下测试，都没有发现移植诱导的对侧感觉注意力不集中的变化。多巴胺能神经支配的背侧新纹状体是能够诱导功能恢复，在许多，但不是所有的，行为测试，涉及侧选择或偏见，不仅在药理学激活后，而且在自发行为的动物。
The ability of embryonic substantia nigra transplants to compensate for behavioral deficits induced by unilateral destruction of the nigrostriatal dopamine pathway was investigated in adult rats. Six days following unilateral 6-OHDA [6-hydroxydopamine] lesions of the nigrostriatal pathway, the adequacy of the lesion was assessed by measurement of the intensity of ipsilateral amphetamine-induced rotation. All rats received surgical cavities in the cortex overlying the head of the caudate-putamen on the lesioned side. In 51 rats, transplants of embryonic substantia nigra were placed on the dorsal surface of the caudate-putamen, and the remaining 19 rats served as unilateral lesioned controls. Behavioral testing was conducted approximately 3 mo. after transplantation: the transplant animals alone showed a marked reduction in ipsilateral rotation induced by 5 mg/kg amphetamine (compensation); although both transplanted and control rats expressed equal contralateral rotation at a dose of 0.25 mg/kg apomorphine, the transplant animals alone showed a marked reduction in rotation at a lower dose of 0.05 mg/kg; the transplanted rats showed less asymmetry in spontaneous rotational behavior than controls, and the asymmetry was further reduced by mild tailpinch; when tested for spontaneous choice behavior in a T-maze, control rats showed 97% selection of the arm ipsilateral to the 6-OHDA lesions; the transplanted rats well compensated on the amphetamine rotation test turned to the contralateral side on 30-40% of choices; no transplant-induced changes were found in contralateral sensory inattention on a sensorimotor test battery, whether tested spontaneously or under mild tailpinch-induced activation. Dopaminergic reinnervation of the dorsal neostriatum is capable of inducing functional recovery in many, but not all, behavioral tests which involve side choice or bias, not only after pharmacological activation but also in the spontaneously behaving animal.