Progressive regulation of sesquiterpene biosynthesis in Arabidopsis and Patchouli (Pogostemon cablin) by the miR156-targeted SPL transcription factors.
Progressive regulation of sesquiterpene biosynthesis in Arabidopsis and Patchouli (Pogostemon cablin) by the miR156-targeted SPL transcription factors.
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DOI：
10.1016/j.molp.2014.11.002
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发表时间：
2014-10
期刊：
影响因子：
27.5
通讯作者：
Zong-Xia Yu;Ling-Jian Wang;Bo Zhao;Chun-Min Shan;Yu-hua Zhang;Dong-Fang Chen;Xiao-Ya Chen
中科院分区：
文献类型：
--
作者：
Zong-Xia Yu;Ling-Jian Wang;Bo Zhao;Chun-Min Shan;Yu-hua Zhang;Dong-Fang Chen;Xiao-Ya Chen
植物代谢产物在其生命周期的不同阶段有所不同。虽然环境因素刺激次生代谢物的生物合成已经得到了很好的证明，但内源发育线索的调节仍然知之甚少。MicroRNA156(MiR156)靶向的Squamosa启动子结合蛋白样(SPL)因子在调节发育阶段转换和开花过程中发挥着重要的年龄信号作用。我们发现，以miR156为靶标的SPL转录因子在倍半萜生物合成的时空调控中起着重要作用。在拟南芥中，miR156-Spl模块通过调节倍半萜合成酶基因TPS21的表达来调节开花期(E)-β-石竹烯的形成。我们证明了SPL9直接与TPS21启动子结合并激活其表达。在多年生芳香草本植物广藿香素中，广藿香油的积累也是受年龄调节的，广藿香油的积累主要由(−)-广霍酚为主的倍半萜组成，并且通过上调广霍香合酶(Patchoulol Synthase，PATPTS)基因的表达来促进老年植物倍半萜的生物合成。由于miR156-SPLs在植物中高度保守，我们的发现不仅揭示了发育时序和倍半萜生产之间的分子联系，而且还建议了一种新的策略，通过提高萜类化合物的生产来工程植物加速生长。
Plant metabolites vary at different stages of their life cycle. Although it is well documented that environmental factors stimulate biosynthesis of secondary metabolites, the regulation by endogenous developmental cues remains poorly understood. The microRNA156 (miR156)-targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) factors function as a major age cue in regulating developmental phase transition and flowering. We show here that the miR156-targeted SPL transcription factor plays an important role in the spatiotemporal regulation of sesquiterpene biosynthesis. InArabidopsis thaliana, the miR156-SPL module regulates the formation of (E)-β-caryophyllene in the flowering stage through modulating expression of the sesquiterpene synthase geneTPS21. We demonstrated that SPL9 directly binds toTPS21promoter and activates its expression. In the perennial fragrant herbPogostemon cablin, the accumulation of patchouli oil, largely composed of sesquiterpenes dominated by (−)-patchoulol, is also age-regulated, and the SPL promotes biosynthesis of sesquiterpenes in elder plants by upregulating patchoulol synthase (PatPTS) gene expression. As miR156-SPLs are highly conserved in plants, our finding not only uncovers a molecular link between developmental timing and sesquiterpene production but also suggests a new strategy to engineer plants for accelerated growth with enhanced production of terpenoids.