Scratch-Wound Assay
Scratch-Wound Assay
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DOI：
10.1007/978-1-61779-207-6_2
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Cory, Giles
中科院分区：
文献类型：
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作者：
Cory, Giles
划痕试验是一种简单、可重复性的试验，通常用于测量基本的细胞迁移参数，如速度、持久性和极性。细胞生长到融合，通过用吸管尖端抓挠而形成一个薄薄的“伤口”。这种方法的优点是，它不需要使用特定的化学诱导剂或梯度小室，它可以产生强烈的定向迁移反应，即使在“单细胞”迁移试验中没有表现出强烈反应的细胞类型中也是如此。当使用延时成像进行分析时，它是最可靠的，这也可以产生有价值的细胞形态/蛋白质定位信息(表1)。
The scratch-wound assay is a simple, reproducible assay commonly used to measure basic cell migration parameters such as speed, persistence, and polarity. Cells are grown to confluence and a thin "wound" introduced by scratching with a pipette tip. Cells at the wound edge polarise and migrate into the wound space.Advantages of this assay are that it does not require the use of specific chemoattractants or gradient chambers and it generates a strong directional migratory response, even in cell types that do not show robust responses in "single cell" migration assays. It is most reliably analysed when performed using time-lapse imaging, which can also yield valuable cell morphology/protein localisation information (Table 1).