Oxidative damage and brain concentrations of free amino acid in chicks exposed to high ambient temperature
Oxidative damage and brain concentrations of free amino acid in chicks exposed to high ambient temperature
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DOI：
10.1016/j.cbpa.2013.12.020
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发表时间：
2014-03-01
影响因子：
2.3
通讯作者：
Furuse, Mitsuhiro
中科院分区：
文献类型：
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作者：
Chowdhury, Vishwajit S.;Tomonaga, Shozo;Furuse, Mitsuhiro
高温可降低雏鸡的采食量和体重，并可引起下丘脑神经肽的表达增加。然而，HT的作用机制以及HT对脑中细胞内稳态的影响还不清楚。在目前的研究中，脂质过氧化和氨基酸代谢的14日龄雏鸡暴露于HT（35摄氏度，24或48小时）或控制热中性温度（CT; 30摄氏度）的大脑中进行了测量。测定脑中丙二醛（MDA）以确定氧化损伤的程度。HT增加体温，减少食物摄入量和体重增加。HT也增加间脑氧化损伤后48小时，并改变了间脑中的一些游离氨基酸浓度。间脑MDA含量随HT和时间的延长而增加，HT的作用随时间的延长而增强。HT改变胱硫醚，丝氨酸，酪氨酸和异亮氨酸的浓度。在HT鸟中，胱硫醚在24小时时比CT鸟低，而在HT鸟中，丝氨酸、酪氨酸和异亮氨酸在48小时时较高。间脑中氧化损伤的增加和氨基酸浓度的改变可能有助于热暴露雏鸡的生理、行为和体温调节反应。(C)2013 Elsevier Inc. All rights reserved.
High ambient temperatures (HT) reduce food intake and body weight in young chickens, and HT can cause increased expression of hypothalamic neuropeptides. The mechanisms by which HT act, and the effects of HT on cellular homeostasis in the brain, are however not well understood. In the current study lipid peroxidation and amino acid metabolism were measured in the brains of 14 d old chicks exposed to HT (35 degrees C for 24- or 48-h) or to control thermoneutral temperature (CT; 30 degrees C). Malondialdehyde (MDA) was measured in the brain to determine the degree of oxidative damage. HT increased body temperature and reduced food intake and body weight gain. HT also increased diencephalic oxidative damage after 48 h, and altered some free amino acid concentrations in the diencephalon. Diencephalic MDA concentrations were increased by HT and time, with the effect of HT more prominent with increasing time. HT altered cystathionine, serine, tyrosine and isoleucine concentrations. Cystathionine was lower in HT birds compared with CT birds at 24 h, whilst serine, tyrosine and isoleucine were higher at 48 h in HT birds. An increase in oxidative damage and alterations in amino acid concentrations in the diencephalon may contribute to the physiological, behavioral and thermoregulatory responses of heat-exposed chicks. (C) 2013 Elsevier Inc. All rights reserved.