Formation of intermediate-conductance calcium-activated potassium channels by interaction of Slack and Slo subunits
Formation of intermediate-conductance calcium-activated potassium channels by interaction of Slack and Slo subunits
复制标题
DOI：
10.1038/2176
复制
发表时间：
1998-10-01
影响因子：
25
通讯作者：
Kaczmarek, LK
中科院分区：
文献类型：
--
作者：
Joiner, WJ;Tang, MD;Kaczmarek, LK
大电导钙活化钾通道（max - k通道）在控制兴奋性和分泌中起着重要作用。目前已知只有一个基因Slo编码对膜电位和细胞内钙敏感的max - k通道。我们已经分离出一种钾通道基因，叫做Slack，它在神经系统中大量表达。松弛通道以25- 65ps的统一电导向外矫正，并受到细胞内钙的抑制。然而，当Slack与Slo共表达时，形成了与Slack和Slo都不匹配的具有药理性质和单通道电导的通道。Slack/Slo通道的中间电导约为60-180 pS，可被细胞质钙激活。我们的研究结果表明，神经系统中的一些中间传导通道可能是由Slack和Slo通道亚基之间的相互作用造成的。
Large-conductance calcium-activated potassium channels (maxi-K channels) have an essential role in the control of excitability and secretion. Only one gene Slo is known to encode maxi-K channels, which are sensitive to both membrane potential and intracellular calcium. We have isolated a potassium channel gene called Slack that is abundantly expressed in the nervous system. Slack channels rectify outwardly with a unitary conductance of about 25-65 pS and are inhibited by intracellular calcium. However, when Slack is co-expressed with Slo, channels with pharmacological properties and single-channel conductances that do not match either Slack or Slo are formed. The Slack/Slo channels have intermediate conductances of about 60-180 pS and are activated by cytoplasmic calcium. Our findings indicate that some intermediate-conductance channels in the nervous system may result from an interaction between Slack and Slo channel subunits.